甘蓝型油菜胞质雄性不育育性恢复基因的分子标记  

Identification of Molecular Markers Linked to the Fertility Restore Gene of CMS in Rapeseed(Brassica napus L.)

作　　者：白红涛[1] 董军刚[1] 薛汉军[1] 董振生[1] 王永行[1] 王一峰[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《西北农业学报》2010年第5期81-85,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省13115项目资助(2007ZDKG-04)

摘　　要：以甘蓝型油菜不育系212A和恢复系1521C组配的含144个单株的F2群体为试验材料,用RAPD和SRAP两种分子标记,结合BSA法,对可育DNA池与不育DNA池筛选,存在差异的引物再用分离群体进行检测。在690条RAPD引物、270对SRAP引物中,获得了与甘蓝型油菜恢复基因Rf连锁的1个RAPD标记OPS11-850和1个SRAP标记M17E13-150,标记与基因Rf之间的遗传距离分别为6.8 c M和10.8 c M。To identify molecular markers closely linked to the CMS restorer gene Rf,RAPD and SRAP technology combined with bulked segregation analysis were used to analyze 144 plants of the F2 population of 212A× the restorer line 1521C.The polymorphic primers were screened between the two DNA pools of male fertile and the male sterile plants,and the selected polymorphic primers were tested in the F2 population.The results showed that one RAPD marker OPS11-850 and one SRAP marker M17E13-150 were identified linked to Rf locus from 690 RAPD primers and 270 SRAP primers.These two molecular markers linked to Rf gene on the same side with a genetic distance of 6.8 cM for OPS11-850 and 10.8 cM for M17E13-150,respectively.

关 键 词：甘蓝型油菜 CMS 恢复基因 RAPD SRAP. 

分 类 号：S565.4[农业科学—作物学]