拟南芥中与ABA信号途径相关的两个同源未知基因的研究  

Study of two unknown homologous genes involved in abscisic acid signaling in Arabidopsis thaliana

作　　者：杨笑玚[1] 吴明杰[1] 王婷婷[1] 蒋彦[1] 李旭锋[1] GRILL E 杨毅[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064 [2]Botanical Institute, University of Munich, Am Hochanger 4, 85354 Freising-Weihenstephan, Germany

出　　处：《四川大学学报（自然科学版）》2010年第3期626-630,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(30971557)

摘　　要：本研究主要在实验室前期筛选出的两个编码同源未知基因AB(AB025622)和AC(AC023628)所构建的拟南芥过量表达和抑制表达转基因植株基础上对转AB基因拟南芥的生理性状进行初步分析以及在转录水平上分AB、AC和ABI1、ABI2在ABA影响下其表达情况。研究证明AB基因的过量表达导致植株对ABA不敏感,失水率增加,而AB的抑制表达导致植株对ABA敏感,降低了植株的失水率。初步证实AB参与ABA信号传导.此外,通过RNA的半定量分析可知在转录水平上AB、AC和ABI1、ABI2的表达量都受ABA诱导变化,证明ABA对野生型拟南芥植株生理性状的影响与控制AB、AC和ABI1、ABI2内源表达量有密切联系.Currently, the physiological characteristics of transgenic plants of AB were analyzed and the alterations of inner expression of AB, AC and ABI1, ABI2 were observed at the transcriptional level under the treatment of AI3A base on the former study that the deregulation transgenic lines of the unknown homologous genes （AB025622 and AC023628） were successfully generated in our laboratory. The results prove that the excessive expression of AB in Arabidopsis lead to the plant insensitive to ABA, increasing the transpiration of plants. However, the suppresses expression of AB cause the plant sensitive to ABA, reducing the transpiration of plants. So, it was confirmed that AB has participated in the ABA signal transduction initially. In addition, the alterations of two unknown gene＇s expressiontionship with alterations of inner expression of AB, AC and ABI1, ABI2.

关 键 词：ABA信号途径 酵母双杂交 半定量分析 

分 类 号：Q78[生物学—分子生物学]