秋水仙素诱导秋花独蒜兰多倍化的研究  

Study on polyploid induction of Pleione maculate Lindl.with colchicine

作　　者：成倩[1] 唐娅梅[1] 张伟[1] 胡晓丽[1] 魏兴强 纳海燕[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]云南英茂生物农业技术公司,安宁650301

出　　处：《四川大学学报（自然科学版）》2010年第3期635-638,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：昆明市科学技术基金项目(昆科技字08G100115)

摘　　要：以秋花独蒜兰(Pleione maculata Lindl.)拟原球茎为材料,研究不同浓度及时间条件下,对秋水仙素诱导秋花独蒜兰多倍化的诱变效果.实验结果表明:秋水仙素浓度为0.2%、处理时间为60 h时,诱导结果最佳,变异率为25.64%;细胞学鉴定表明,该方法诱导培育的植株具有多倍体表型特征,其体细胞染色体数多为2n=76～80条,而对照2n=38～40条.The protocorm like bodies （PLBs） of Pleione maculate Lindl. were used as explants for the polyploid induction with different densities of colchicines. The results were stated as follows： the highest morphological variation rate is 25.64% by 0.2% colchicines concentrations treated 60 h. Observing the chromosomes of P. rnaculate microscopically, the chromosome number of tetraploid cells was 2n=38 -40, while that of diploid cells was 2n=76-80.

关 键 词：秋花独蒜兰 拟原球茎 秋水仙素处理 染色体数 

分 类 号：Q343.2[生物学—遗传学]