脂多糖对人近曲肾小管上皮细胞表面活性蛋白D及肿瘤坏死因子-α表达的影响  

Effects of 1ipopolysaccharide on the expressions of surfactant protein D and tumor necrosis factor-alpha in human renal proximal tubular epithelial cells

作　　者：胡凤琪[1] 丁国华[1] 梁伟[1] 刘娇[1] 任志龙[1] 

机构地区：[1]武汉大学人民医院肾内科,430060

出　　处：《实用医学杂志》2010年第10期1710-1712,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:30670985)

摘　　要：目的:探讨脂多糖(1ipopolysaccharide,LPS)对人近曲肾小管上皮细胞(HK-2)表面活性蛋白D(surfactant protein D,SP-D)及肿瘤坏死因子(TNF-α)表达的影响。方法:将体外培养HK-2细胞分为5组,分别给与0、0.1、1、5、10μg/mL LPS刺激8h。免疫印迹和RT-PCR观察SP-D蛋白及mRNA表达变化,ELISA和RT-PCR观察TNF-α蛋白和mRNA表达变化。结果:1、5、10μg/mL LPS刺激8h可引起HK-2细胞SP-D蛋白及mRNA表达显著降低(P<0.05),1、5、10μg/mL LPS刺激8h可引起HK-2细胞TNF-α蛋白及mRNA表达显著增加(P<0.05)。SP-D与TNF-α的表达呈负相关。结论:LPS刺激下TNF-α表达增加与SP-D的表达降低有关。SP-D可能在肾脏炎症反应中发挥重要作用。Objective To investigate the effects of 1ipopolysaccharide （LPS） on the expressions of surfactant protein D （SP-D） and TNF-α in human renal proximal tubular epithelial cells （HK-2）. Methods HK-2 cells were treated with LPS at various concentrations（0,0.1,1,5 and 10 μg / mL） for 8 h. Expression of SP-D was detected by Western blot and RT-PCR and that of TNF-α was determined by ELISA and RT-PCR. Results The expression levels of SP-D mRNA and protein in HK-2 cells significantly decreased after treatment with LPS at 1,5,and 10 μg / mL （P 0.05） while those of TNF-α mRNA and protein increased remarkably （P 0.05）. The expression of SP-D was negatively associated with that of TNF-α. Conclusions With LPS stimulation,an increase in the expression of TNF-α is associated with a decrease in the expression of SP-D. SP-D may play an important role in the modulation of renal inflammation.

关 键 词：脂多糖类 表面活性蛋白D 肿瘤坏死因子Α 

分 类 号：R692[医药卫生—泌尿科学]