蕨麻多糖抗缺氧作用研究  

The anti-hypoxia effects of potentilla anserine L. polysaccharide

作　　者：韦薇[1] 李广策[2] 龚海英[2] 李怡[2] 李建宇[2] 李灵芝[2,3] 

机构地区：[1]武警医学院救援医学教研室,天津300162 [2]武警医学院药物化学教研室,天津300162 [3]天津市职业与环境危害生物标志物重点实验室,天津300162

出　　处：《武警医学院学报》2010年第5期345-347,373,共4页Acta Academiae Medicinae CPAPF

基　　金：国家自然科学基金项目(30672774);天津市重点课题(10JCZDJC21100)

摘　　要：【目的】研究蕨麻多糖抗缺氧作用。【方法】取新生SD乳鼠心肌细胞,建立缺氧损伤模型,设正常对照组、缺氧损伤模型组、香丹注射液阳性对照组(2mg/ml)、蕨麻多糖高、中、低浓度组,浓度分别为0.2、0.02、0.002mg/ml。正常对照组在DMEM/F-12培养基中37℃培养于含5%CO2培养箱中,其它各组在D-Hanks液中37℃在混合气体培养箱中(95%N2、5%CO2、O2浓度<1%)。培养6h后,采用四甲基偶氮唑盐法检测心肌细胞代谢活力;采用比色法检测细胞外乳酸脱氢酶活性。采用小鼠常压窒息性缺氧模型,观察缺氧状态下各组小鼠的存活时间。【结果】与缺氧模型组比较,蕨麻多糖0.2mg/ml、0.02mg/ml剂量组均使细胞代谢率显著升高(P<0.05)。与正常对照组比较,给予小鼠蕨麻多糖1.8g/kg可使其平均存活时间延长28.91%(P<0.01)。【结论】蕨麻多糖具有抗缺氧作用,并与剂量有关。[Objective] To study the anti-hypoxia and radical scavenge effects of potentilla anserine L. polysaccharide. [Methods] A model of hypoxia injury was developed by using primary cultural myocytes of neonatal SD rats. The metabolic ability of the cells treated with potentilla anserine L. polysaccharide was detected by methyl thiazolyl tetrazolium （MTT） assay. The activities of lactate dehydrogenase（LDH） out of cells were measured by spectrophotometry. The model of normobaric hypoxia was used for studying the effect of potentilla anserine L. polysaccharide on the average survival time of mice. [Results] After treated with potentilla anserine L polysaccharide of 0.2 mg/ml or 0.02 mg/ml, the metabolic ability of the myocytes incresead significantly when compared with the model group after 6 h of hypoxia. After treated with potentilla anserine L. polysaccharide of 1.8 g/kg, the average survival time of mice was prolonged by 28.91 % when compared with the control group and the difference was statistically significant. [Conclusions] The Potentilla anserina L. polysaccharide shows anti-hypoxia ability and dose dependant.

关 键 词：蕨麻多糖 缺氧 心肌细胞 

分 类 号：Q593.3[生物学—生物化学]