Xylazine+DHE合剂对大鼠的ED_(50)值测定及剂量确定  

Determination of ED 50 Values and Dosages of xylazine and Dihydroeterphine Complex on Rats

作　　者：张立树[1] 闫章年[1] 李东郊[1] 田慧英[1] 

机构地区：[1]解放军农牧大学军事兽医研究所

出　　处：《黑龙江畜牧兽医》1999年第2期1-3,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金

摘　　要：以翻正反射消失和痛阈增加３０％为指标，应用序贯法测定ｘｙｌａｚｉｎｅ＋ＤＨＥ合剂腹腔给药对大鼠的麻醉半数有效量（ａｎｅｓｔｈｅｔｉｃＥＤ５０）和镇痛半数有效量（ａｎａｌｇｅｓｉｃＥＤ５０）；在此基础上，确定给药剂量并检验给药剂量是否合理。结果表明，ｘｙｌａｚｉｎｅ＋ＤＨＥ合剂对大鼠的麻醉ＥＤ５０为８．３６４ｍｇ／ｋｇ，镇痛ＥＤ５０为７．８０２ｍｇ／ｋｇ；确定麻醉剂量为１５．００ｍｇ／ｋｇ，镇痛剂量为１０．０００ｍｇ／ｋｇ。进一步的麻醉和镇痛实验显示，麻醉剂量使９３．３％大鼠的翻正反射消失，镇痛剂量使大鼠的摆尾阈值增加幅度在４０％～６０％，且维持时间在６０ｍｉｎ左右，表明这两个剂量完全符合实验要求。With the disappearance of righting reflex and tail-flicklatency greater than 30% as indicators,we determined the anesthetic andanalgesic ED 50 of xylazine and dihydroeterphine(DHE)complex in ratsintraperitoneally with sequential analysis.The dosages were determinedthen on the basis of the two ED 50 values,and a support test was carriedout to decide whether the dosages were optional.The results showed thatthe anesthetic and analgesic ED 50 were 8.364 and 7.802 mg/kg,respectively.And the dosages determined according to the ED 50 were 15.000 and 10.000 mg/kg,respectively.The support test showed that theanethetic dosage made 93.3% rats' righting reflex disappear,and thealgethetic dosage the tail-flick latency greater than 50% and last forabout 1 hour,which thoroughly satire for further exmperimental requirements.

关 键 词：序贯法 麻醉半数有效量 腹腔注射 大鼠 

分 类 号：S859.791[农业科学—临床兽医学]