垂枝桦组织培养技术研究  

Study on tissue culture technology of Betula pendula

作　　者：李国福 陈士刚[2] 秦彩云[2] 任皎[2] 陶晶[2] 

机构地区：[1]吉林省林木种子调制储备中心,吉林长春130607 [2]吉林省林业科学研究院,吉林长春130033

出　　处：《吉林林业科技》2010年第3期1-3,共3页Journal of Jilin Forestry Science and Technology

摘　　要：对垂枝桦芽茎段组织培养技术的外植体启动培养、丛生芽增殖以及试管苗生根等方面开展研究。结果表明:在1/2MS+6-BA1.00 mg.L-1+NAA0.05 mg.L-1+蔗糖30 g.L-1的培养基上,外植体诱导率最高;在1/2MS+6-BA0.20 mg.L-1+NAA1.50 mg.L-1+蔗糖30 g.L-1的培养基上,外植体增殖系数最高,可达5～8倍;在1/2MS+NAA0.50 mg.L-1+蔗糖20 g.L-1的培养基中,继代苗的生根率可达100.0%。This article studied the tissue culture of Betula pendula,including inducing callus,buds multiplication and radication of tube seedlings in different culture mediums.The result showed that the highest inducement occurred in culture medium with 1/2MS＋6-BA1.00 mg·L-1＋NAA0.05 mg· L-1＋30 g·L-1saccharose;the highest bud multiplication occurred in culture medium with 1/2MS＋6-BA0.20mg·L-1＋NAA1.50 mg·L-1＋30g·L-1 saccharose,at where the multiplication coefficent was up to 5～8;the highest rooting rate occurred in culture medium with 1/2MS＋NAA0.50 mg·L-1＋20 g·L-1saccharose culture medium,and the rooting ratio could reach to 100.0%.

关 键 词：垂枝桦 组织培养 无性繁殖 

分 类 号：S722.37[农业科学—林木遗传育种]