1，3-丁二烯致染色体损伤的遗传易感性与CYP2E1和GSTs基因多态性关系  

Study on the Relationship between Chromosomal Damage and Polymorphisms of Metabolizing Enzymes in 1,3-butadiene-exposed Workers

作　　者：谭红汕[1] 王琪[1] 王爱红[2] 冯楠楠[1] 叶云杰[1] 冯晓青[3] 郑玉新[4] 夏昭林[1] 

机构地区：[1]复旦大学公共卫生学院,上海200032 [2]浙江省宁波市疾病预防控制中心,浙江宁波315834 [3]苏州大学公共卫生学院,江苏苏州215000 [4]中国疾病预防控制中心职业卫生与中毒控制所,北京100000

出　　处：《环境与职业医学》2010年第5期266-269,共4页Journal of Environmental and Occupational Medicine

基　　金：科技部十一五支撑计划(编号：2006BA106B02);上海市公共卫生重点学科建设计划(编号：08GW2X0402)

摘　　要：[目的]探讨代谢酶基因CYP2E1和GSTs的基因多态性与1,3-丁二烯致外周血淋巴细胞染色体损伤易感性的关系。[方法]采用胞质分裂阻滞微核试验方法(CBMN)评价166名丁二烯接触工人和41名对照组染色体损伤水平,应用PCR-RFLP测CYP2E1c1c2基因多态,PCR法测GSTT1和GSTM1缺失情况。[结果]接触组和对照组的微核发生率分别为(3.23±2.49)‰和(1.22±1.19)‰,差别有统计学意义(P<0.01)。多因素Poisson回归分析发现CYP2E1c1c2/c2c2基因型和GSTM1非缺失型与染色体损伤相关(分别为χ2=14.39,P<0.01和χ2=4.23,P<0.05)。没有发现年龄、工龄、性别、吸烟和饮酒等与微核率之间的关系。[结论]双核淋巴细胞微核数可以作为1,3-丁二烯接触早期健康损害的指标。CYP2E1c1c2/c2c2基因型、GSTM1非缺失型与1,3-丁二烯诱导的染色体损伤有关。[ Objective ] To explore the relationship between chromosomal damage induced by 1,3-butadiene （ BD ） and polymorphisms of metabolizing enzymes of CYP2E1 and GSTs. [ Methods ] Cytokinesis-block micronucleus （ CBMN ） assay was used to detect chromosomal damage in peripheral lymphocytes of 166 1,3-butadiene exposed workers and 41 controls. PCR-RFLP technique was applied to detect polymorphisms in CYP2E1 clc2. Null genotypes of GSTT1 and GSTMI were detected by PCR. [ Results ] Compared the CBMN frequencies of the two groups we found that the frequency in exposed workers was higher than that in control group（ P〈0.01 ）. Individuals with the CYP2E1 c1c2/c2c2 and GSTMI positive genotype were observed to have significantly higher CBMN frequencies than individuals with more common genotypes （Z~=14.39, P〈0.01 and χ^2=4.23, P〈0.05 ）. Sex, age, duration of work, smoking and drinking habit had no relationship with CBMN frequencies. [ Conclusion ] BD-exposed workers had higher risk of chromosomal damage compared with control. Cytokinesis-block micronucleus （ CBMN ） assay can be used to evaluate the early damage of butadiene-exposed workers. CYP2E1 c1c2/c2c2 and GSTMI positive genotype showed higher average CBMN frequencies than those with more common genotypes.

关 键 词：1 3-丁二烯 染色体损伤 细胞色素P4502E1基因 谷胱甘肽s-转移酶基因 基因多态 

分 类 号：R114[医药卫生—卫生毒理学]