镉致大鼠前体精原干细胞-支持细胞共培养系损伤的形态变化  

Morphological Change Induced by Cadmium in Primary Rat Gonocyte Cell—Sertoli Co-cultures in vitro

作　　者：赖纯米[1] 张杰[2] 吴庆[2] 李克勇[2] 周志俊[2] 常秀丽[2] 

机构地区：[1]云南医学高等专科学校,云南昆明650000 [2]复旦大学公共卫生学院、公共卫生安全教育部重点实验室,上海200032

出　　处：《环境与职业医学》2010年第5期280-283,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金(编号:30671728,20977019);上海市卫生局专项基金(编号:08GWZX0303)

摘　　要：[目的]通过对大鼠前体精原干细胞-支持细胞共培养系进行镉染毒,探讨镉对前体精原干细胞和支持细胞共培养系早期效应的形态学变化。[方法]从3天龄的SD大鼠睾丸中分离前体精原干细胞和支持细胞并进行24h共培养后,再分别给予0、2.5、5.0、10.0、20.0μmol/L的氯化镉(CdCl2)染毒12h,然后用碱性磷酸酶和油红O染色分别鉴定前体精原干细胞和支持细胞,观察不同细胞的形态,并用原位末端标识法(TUNEL方法)进行细胞凋亡的原位检测。[结果]2.5μmol/LCdCl2染毒未显著改变共培养中精原干细胞和支持细胞的形态。5.0μmol/L以上浓度CdCl2染毒可致支持细胞(由油红O染色确认)的结构呈现明显变化,具有浓度依赖趋势,用TUNEL法检测发现前体精原干细胞和支持细胞均发生凋亡,和对照组相比差别有统计学意义。[结论]CdCl2染毒12h可诱导支持细胞的形态改变,以及前体精原干细胞和支持细胞的凋亡。[ Objective ] To investigate the acute effects of cadmium on primary rat gonocyte cell/sertoli co-cultures. [ Methods ] Gonocytes and sertoli cells were separated from 3-day SD rat. Cadmium（ 0, 2.5, 5, 10, 20 p.mol/L ）were added to coculture system 12h after gonocyte and sertoli celt had co-cultured for 24h. Alkaline phosphatase and oilred O were used to identify the gonocyte and sertoli cells. TUNEL in situ detection was used for detecting apoptosis. [ Results ] Exposure of 2.5 μmol/L cadmium did not induce morphologic change of gonocytes and sertoli cells. When exposure dose greater than 5 μmol/L, sertoli cells showed morphological change identified by oilred O in a dose-dependent manner. TUNEL in situ detection suggested that cadmium increased apoptosis in both gonocytes and sertoli cells. [ Conclusion ] Exposure of cadmium for 12 h induced morphological change of sertoli cells and apoptosis of both gonocyte and sertoli cell.

关 键 词：前体精原干细胞 支持细胞 共培养 镉 

分 类 号：R114[医药卫生—卫生毒理学]