HSP70-1、HSP70-2基因多态性与肺癌易感性的相关性  

The Heat Shock Protein HSP70-1 and HSP70-2 Gene Polymorphism and Susceptibility to Lung Cancer

作　　者：王玉珍[1] 吴逸明[1] 周舫[1] 李伟辉[1] 梁守沛[1] 许东[1] 任文杰[1] 

机构地区：[1]郑州大学公共卫生学院劳动卫生学教研室,河南郑州450001

出　　处：《环境与职业医学》2010年第5期305-309,共5页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助项目(编号:30600490);河南省教育厅科技创新人才支持计划(编号:2008HASTIT028)及河南省教育厅自然科学研究资助项目(编号:2008A320018)

摘　　要：[目的]探讨热休克蛋白HSP70-1+190(G/C)、HSP70-2+1267(A/G)两位点基因多态性与肺癌易感性的相关性。[方法]采用病例-对照研究,以病理确诊的肺癌患者159例为病例组,202名正常体检人群为对照组。应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)技术检测HSP70-1+190(G/C)和HSP70-2+1267(A/G)的单核苷酸多态性。采用非条件Logistic回归分析两位点基因型与吸烟的交互作用及两位点之间的交互作用。[结果]HSP70-1+190(G/C)位点的3种基因型GG、GC和CC及G和C等位基因频率在病例组和对照组之间的分布,差异均有统计学意义(χ2=19.16,P<0.05;χ2=18.76,P<0.05),CC基因型与肺癌具有中等强度关联。HSP70-2+1267(A/G)位点的基因型及等位基因的分布在两组之间差异均无统计学意义(P>0.05)。按照吸烟情况进行分层分析发现,重度吸烟人群中携带HSP70-1+190(G/C)位点的GC基因型和GC+CC基因型者及HSP70-2+1267(A/G)位点的AA基因型的个体患肺癌的风险增高。基因型与吸烟的交互作用分析发现,HSP70-1+190(G/C)位点多态性和吸烟具有相乘交互作用(P=0.01)。HSP70-1+190(G/C)和HSP70-2+1267(A/G)两位点多态性之间存在交互作用(χ2=12.87,P=0.00)。[结论]HSP70-1+190(G/C)位点多态性可能与河南汉族人群中肺癌易感性有关,与HSP70-2+1267(A/G)和吸烟均具有相乘交互作用;HSP70-2+1267(A/G)位点多态性与肺癌的相关性还有待进一步研究,其AA基因型可能与重度吸烟之间存在交互作用。[ Objective ] To investigate the association between the heat shock protein 70 gene polymorphism and lung cancer. [ Methods ] A case-control study was conducted among 159 lung cancer patients and 202 control subjects. Polymerase chain reaction-restrained fragment length polymorphism technique （ PCR-RFLP ） was applied to detect the single nucleotide polymorphisms （ SNPs ） of HSP70-1 ＋190 （ G/C ） and HSP70-2 ＋1267 （ A/G ）. The interactions of the two gene polymorphisms and smoking and that of the two sites were analyzed by non-conditional Logistic regression. [ Results ] There were significant differences between the case group and control group（χ^2= 19.16, P 〈 0.05;χ^2 = 18.76, P 〈 0.05 ）in the distribution of each genotypes GG, GC and CC, and also in the frequencies of the two alleles G and C of HSPTO-1 ＋190（ G/C ）. The CC genotype was moderately correlated with lung cancer. There were no significant differences in the distribution of genotypes and allele frequencies of HSP70-2 ＋ 1267 （ A/G ） between the case group and control group （ P 〉 0.05 ）. After stratification analysis according to smoking, it revealed that in heavysmokers subgroups who carried HSP70-1 ＋190（ G/C ）GC or GC＋CC genotypes and/or HSP70-2 ＋1267（ A/G ）AA genotype were associated with significantly increased risk of lung cancer. The interaction analysis of genotype and smoking revealed that there was multiplication interaction between HSP70-1 ＋190 （ G/C ）polymorphism and smoking on susceptibility to lung cancer. There was also interaction between the two genotypes HSP70-1 ＋190（ G/C ）and HSP70-2 ＋1267（ A/G χ^2 = 12.87, P = 0.00 ）. [ Conclusion ] HSP70-] ＋190（ G/C ）polymorphism may be associated with the susceptibility to lung cancer in He＇nan Han population, and there are multiplication interaction with HSP70-2 ＋1267 （A/G）polymorphism and smoking. The relationship between HSP70-2 ＋1267 （A/G） polymorphism and lung cancer has yet to be further studied

关 键 词：热休克蛋白70 单核苷酸多态性 肺癌 易感性 基因多态性 

分 类 号：R181.3[医药卫生—流行病学]