梅PGIP基因的启动子克隆及生物信息学分析  

Cloning and Sequence Analyzing of PGIP Promoter from Prunus mume

作　　者：李广平[1,2] 张长青[3] 章镇[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]南京林业大学森林资源与环境学院,南京210037 [3]金陵科技学院园艺系,南京210038

出　　处：《西北植物学报》2010年第5期883-887,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30671438)

摘　　要：根据梅PGIP基因（GenBank登录号：DQ364056.1）5′端序列设计特异反向引物,利用染色体步行法获得了该基因上游1 037 bp的启动子序列.启动子结构分析表明,梅PGIP基因启动子序列与中国李PGIP基因启动子显著相似,相似度达89%~96%,较中国李PGIP基因启动子多一个100 bp的区段,与水稻和豆的启动子序列均无Blast比对结果.转录调控元件预测结果表明,克隆序列与豆相应序列的保守区存在着能调控抗病基因转录的GT1结合位点.Using chromosome walking method,we have cloned the PGIP promoter with 1 037 bp length from Prunus mume,and the primer is designed reversely according to 5′ terminal sequence of PGIP gene from P.mume（GenBank accession number：DQ364056.1）.Sequence structure analysis showed that the similarity of PGIP gene promoter was high to 89%~96% between P.mume and Prunus salicina,and there was an additional segment with 100 bp length in the PGIP gene promoter from P.mume,but the similarity was so little that there was no blast result between P.mume and Phaseolus vulgaris and Oryza sativa.Transcriptional regulatory elements prediction results showed that there existed a GT1 binding site which regulated transcription of resistance gene in conservative region between P.mume and Phaseolus vulgaris.As a result,it provided a theoretical basis for revealing transcription regulation mechanism of PGIP gene from P.mume.

关 键 词：梅 PGIP基因 启动子 调控元件 

分 类 号：Q789[生物学—分子生物学]