辣椒ML基因植物表达载体的构建及其转化  

Construction and Transformation of the Plant Expression Vector Carrying ML Gene of Pepper

作　　者：包良帅[1] 巩振辉[1] 李大伟[1] 黄炜[1] 逯明辉[1] 陈儒钢[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100

出　　处：《西北植物学报》2010年第5期901-904,共4页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30571262;30771467);"十一五"国家科技支撑计划(2006BAD01A7);"13115"科技创新工程重大科技专项(2008ZDKG-03);教育部高校博士点基金(200807120007);国家高技术研究发展计划(2009AA10Z104-6)

摘　　要：为分析辣椒ML基因在抗辣椒疫病方面的作用,构建了ML基因的植物表达载体pBI121-ML,采用快速冻融法将表达载体导入农杆菌EHA105,并用农杆菌介导法转化辣椒感病品种B12,对得到的转化植株经PCR和RT-PCR分子检测,结果显示,获得了4个辣椒转基因株系.In order to analyse the effect of ML gene to Phytophthora capsici,plant expression vector pBI121-ML was constructed by using the vector pBI121.The expressed vector pBI121-ML was transferred into Agrobacterium tumefaciens EHA105 by means of rapid frozen thaw method successfully.Pepper susceptible Cv.B12 was transformed with pBI121-ML by Agrobacterium tumefaciens-mediated transformation.PCR and RT-PCR detection confirmed that four transgenic lines of pepper were obtained.

关 键 词：辣椒 ML基因 植物表达载体 遗传转化 

分 类 号：Q789[生物学—分子生物学]