骨髓间充质干细胞对造血干细胞“干性”的保持作用  

Effect of bone marrow-derived mesenchymal stem cells on keeping the stemness of hematopoietic stem cells

作　　者：施小凤[1] 尤海燕[1] 巴荣[1] 朱彦[1] 费小明[1] 陆益龙[1] 罗敏[1] 庄琴[1] 余先球[1] 王丽霞[1] 张广莲[1] 

机构地区：[1]江苏大学附属医院血液病科,镇江212001

出　　处：《江苏医药》2010年第10期1178-1180,I0001,共4页Jiangsu Medical Journal

基　　金：江苏大学临床医学科技发展基金(JLY2050028)

摘　　要：目的探讨骨髓间充质干细胞(MSCs)对造血干细胞(HSCs)"干性"的保持作用及其机制。方法免疫磁珠分离纯化脐血HSCs-CD34+细胞,并分组培养:A组,CD34+细胞单独培养;B组,加细胞因子;C组,加细胞因子和MSCs(直接接触);D组,加细胞因子和MSCs(不接触);E组,加MSCs(直接接触);F组,加MSCs(不接触)。0、7、14d分别计扩增倍数、单位细胞数形成集落能力、CD34+比率,计数7d时各组CD34+、CD34+CD38-比率。结果随着时间延长扩增倍数增加(尤其B组),形成集落的能力和CD34+细胞比率渐下降,但E、F组下降不明显。结论以MSCs为滋养层,对HSCs扩增作用不明显,但可更好地保持HSCs的"干性";这种保持"干性"的作用依赖于细胞与细胞间的相互黏附作用。Objective To investigate the effect of bone marrow-derived mesenchymal stem cells（MSCs） on keeping the stemness of hematopoietic stem cells（HSCs）.Methods CD34＋ cells were enriched with immuno-magnetic microbeads and divided into six groups in different cultivating mediums of CD34＋ cells alone（group A）,adding growth factors （group B）,adding growth factors and direct contact with MSCs feeder layer（group C）,adding growth factors and indirect contact with MSCs feeder layer（group D）,direct contact with MSCs feeder layer（group E）,and indirect contact with MSCs feeder layer（group F）.After cultivated for 0,7 or 14 days,the outputs of total cells,CD34＋ and CD34＋CD38-ratio and colony forming units were evaluated at different times.ResultsAs time passing by,although the number of the total cell increased,specially in group B,the ability of forming CFU and the ratio of CD34＋ decreased gradually except for groups of E and F.Conclusion In cultivating on MSCs feeder layer without growth factors,expansion of HSCs was not obvious,but the ＂stemness ＂ of HSCs could be kept well.This process mainly depends on the cell-cell direct adhesion.

关 键 词：造血干细胞 骨髓间充质干细胞 干性 

分 类 号：R331[医药卫生—人体生理学]