七叶皂苷钠对ox-LDL诱导的U937细胞表达炎症因子的影响  

Effects of Sodium Aescinate on the Expression of Inflammatory Factor Induced by Oxidize Low-Density Lipoprotein (ox-LDL) in U937 Cells

作　　者：王秀玲[1] 魏欣冰[1] 赵云雪[1] 丁华[1] 傅风华[2] 

机构地区：[1]山东大学医学院药理研究所,济南250012 [2]烟台大学药学院,山东烟台264005

出　　处：《中国药学杂志》2010年第10期743-746,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30772760)

摘　　要：目的研究七叶皂苷钠对氧化低密度脂蛋白(ox-LDL)诱导的人单核细胞系U937细胞所产生的一氧化氮(NO)以及对肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)表达的影响。方法将培养的U937细胞分为正常细胞对照组、ox-LDL组和七叶皂苷钠(20、10、5μg.mL-1)药物干预组。ox-LDL组是将U937细胞与40mg.L-1的ox-LDL共同孵育24h;药物干预组是将不同质量浓度的七叶皂苷钠与40mg.L-1的ox-LDL同时加入培养的U937细胞,共同孵育24h。应用硝酸还原酶法测定NO的分泌量,酶联免疫吸附法测定TNF-α和IL-6的蛋白表达。结果 ox-LDL可刺激U937细胞分泌NO,并促使TNF-α和IL-6的表达增加;20、10μg.mL-1的七叶皂苷钠能够降低ox-LDL诱导的上述炎症因子的升高。结论七叶皂苷钠能抑制ox-LDL诱导的U937细胞表达NO、TNF-α和IL-6。OBJECTIVE To investigate the effects of sodium aescinate on the oxidize low-density-induced increases in nitric oxide （NO） production and the expression of inflammatory factors in the U937 cells.METHODS U937 cells were divided into 5 groups：normal group,ox-LDL group （U937 cells were incubated with 40 mg·L^-1 ox-LDL for 24h）,treatment groups with sodium aescinate （5,10,20 μg·mL^-1）（ U937 cells were incubated with sodium aescinate and 40 mg·L^-1 ox-LDL for 24 h）.Then the production of NO in medium was measured by nitrate reductase.The expressions of tumor necrosis factor-α （TNF-α） and interleukin-6（IL-6） in medium were detected by enzyme linked immunosorbent assay （ELISA）.RESULTS In the ox-LDL group,the production of NO and the expression of TNF-α and IL-6 were significantly increased compared with normal group.However,sodium aescinate （20,10 μg·mL^-1） treatment blocked ox-LDL-induced increases in NO,TNF-α and IL-6.CONCLUSION Sodium aescinate is could attenuate the NO production,expressions of TNF-α and IL-6 induced by ox-LDL in U937 monocyte cells,according to suppresses inflammatory reactions.

关 键 词：七叶皂苷钠 U937细胞 一氧化氮 肿瘤坏死因子-α 白细胞介素-6 

分 类 号：R965[医药卫生—药理学]