外翻肠囊法快速发现吴茱萸汤吸收成分群的研究  

Everted intestinal sac method for quick finding absorption ingredients of Wuzhuyu decotion

作　　者：龚慕辛[1,2] 王雅璕[1] 宋亚芳[2] 王智民[2] 张启伟[2] 王维皓[2] 朱晶晶[2] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2010年第11期1399-1404,共6页China Journal of Chinese Materia Medica

基　　金：国家"十一五"支撑计划项目(2006BAI08B03);2007年中医药行业科技专项项目(200707007;200707009)

摘　　要：目的：建立快速发现吴茱萸汤主要吸收成分的外翻肠囊模型法,为选择吴茱萸汤质量控制指标提供参考。方法：采用大鼠外翻肠囊模型,收集3种浓度的吴茱萸汤给药后不同时间的肠囊液样品,用HPLC检测样品中的吴茱萸碱（evodi-amine,Ev）、吴茱萸次碱（rutaecarpine,Ru）、吴茱萸内酯（limonin,Li）、人参皂苷Rb1,Rg1,Re（ginsenoside-Rb1,-Rg1,-Re,Rb1,Rg1,Re）、异鼠李素-3-O-芸香糖苷[isorhamnetin-3-O-β-D-glucosyl（6″→1″′）-α-L-rhamnoside,Irs]、姜辣素（6-gingerol,6-Gi）,计算累计吸收量,同时比较吴茱萸汤样品与吸收成分间比例。结果：除Ru未检出外,其余7种成分均可进入肠囊,但高浓度汤中Ev在空肠和回肠段中未检出,中浓度仅在空肠的150 min后检出,而低浓度则在60 min后的时间点均检出。不同浓度吴茱萸汤吸收趋于稳定后的成分间比例与原汤中各成分间比例不同。结论：肠囊对药物成分吸收有选择性,空肠与回肠相比可更多、更快地给出吸收成分信息,选择外翻肠囊法90~150 min的样品检测吴茱萸汤吸收成分比较适宜。Objective： To establish a method for quick finding the absorption ingredients of Wuzhuyu decotion in order to select the index to control its quality.Method： The absorption of three concentration of Wuzhuyu decotion was investigated with the in vitro-everted intestinal sac model.The intestinal bag fluid of jejunum and ileum were collected in different time and the eight ingredients,which were evodiamine（Ev）,rutaecarpine（Ru）,limonin（Li）,ginsenoside-Rb1,-Rg1,-Re（Rb1,Rg1,Re）,isorhamnetin-3-O-β-D-glucosyl（6″→1）-α-L-rhamnoside（Irs）and 6-gingerol（6-Gi）,were detected by HPLC as the represent constituents in samples.Result： Eight ingredients except Ru in samples could be detected,but Ev could not be detected in high concentration samples.The ratios between absorption ingredients were different from in Wuzhuyu decotion.Conclusion： The in vitro-everted intestinal sac can absorb the ingredients of Wuzhuyu decotion selectivity.Compare with the ileum,the jejunum can provide the more absorption information and faster,the best test time is 60-90 min.

关 键 词：吴茱萸汤 外翻肠囊模型 HPLC 吸收成分检测 

分 类 号：R283[医药卫生—中药学]