反复离心运动对大鼠骨骼肌损伤和蛋白质降解机制的影响  

Effects of Repeated Eccentric Exercise on Skeletal Muscle Injury and the Mechanism of Proteolysis in Rat

作　　者：金其贯[1] 刘霞[1] 李淑艳[1] 常永娜[1] 李宁川[1] 

机构地区：[1]扬州大学体育学院,江苏扬州225002

出　　处：《体育科学》2010年第6期76-80,共5页China Sport Science

基　　金：国家体育总局应用基础研究项目(IK0408208)

摘　　要：目的:探讨连续离心运动训练对骨骼肌中Calpain和Ubiquitin含量的影响及其与骨骼肌损伤之间的关系;方法:雄性SD大鼠29只随机分为对照组、离心训练后即刻组、24h组和7天组。训练组大鼠连续进行7天的下坡跑运动,分别在末次训练后即刻,24h和第7天取股四头肌,测定股四头肌的超微结构、血清LDH和CK活性以及骨骼肌中Calpain-1、Calpain-2和Ubiquitin含量;结果:1)7天离心训练后即刻和24h,股四头肌超微结构的损伤性变化呈渐进性加重,离心训练后24h组出现明显的肌丝坏死。训练后第7天仍不能完全恢复。血清CK和LDH活性的变化与大鼠股四头肌超微结构的变化相一致。2)与对照组相比,末次训练后即刻骨骼肌中Calpain-1、Calpain-2和Ubiquitin含量均显著升高;训练后24h,Calpain-1和Calpain-2含量进一步升高,其中,Calpain-1显著高于对照组和训练后24h组,Calpain-2虽显著高于对照组,但与训练后即刻组相比无显著性差异;而Ubiquitin含量显著低于训练后即刻组。训练后第7天,Calpain-1和Calpain-2含量显著低于训练后24h组,但仍然高于对照组;而Ubiquitin含量虽高于训练后24h组和对照组,但无显著性差异;结论:1)连续的离心运动可能对骨骼肌纤维的损伤产生一定的累加作用;2)短期连续的离心训练后,骨骼肌中Calpain和Ubiquitin的动态变化几乎与骨骼肌超微结构的动态变化相一致。离心运动训练导致骨骼肌中Calpain和Ubiquitin含量的增加,可能是导致骨骼肌累积性损伤的重要机制。Objective：To investigate the effects of continuous eccentric exercise training on Calpain and Ubiquitin content skeletal muscle and its relationship with skeletal muscle injury.Methods：29 male SD rats were randomly divided into control group,immediately post-training group（ET0）,24h post-training group（ET24）and 7 days post-training group（ET7d）.Training rats consecutive downhill running for 7 days.Quadriceps were taken immediately,24h and 7th day after the last bout of eccentric exercise respectively,the changes of ultrastructure,serum LDH and CK activity and Calpain-1,calpain-2,Ubiquitin concentrations were observed.Results：1）Ultrastructural changes of quadriceps injury showed gradual increase immediately and 24h at the last bout of eccentric exercise,the apparent necrosis of myofilament occurred at 24h post-training.It still not fully recovered at 7th day after training.The changes of serum CK and LDH activity had consistent with ultrastructural changes.2）Compared with the control group,Calpain-1,Calpain-2 and Ubiquitin levels in skeletal muscle were significantly increased immediately after the last training.At 24h post-training,Calpain-1 and Calpain-2 levels further increased,including Calpain-1 significantly higher than that in control group and ET0 group,Calpain-2 significantly higher than in control group,but there was no significant difference compared with ET0 group,while Ubiquitin content was significantly lower than that in ET0 group.At the 7th post-training,Caipain-1 and Calpain-2 were significantly lower than those in ET24 group,but still higher than control group.While Ubiquitin levels in ET7d group was higher than that in ET24 group and the control group,but there were no significant difference.Conclusion：1）Repeated eccentric exercise might produce a certain cumulative effect on skeletal muscle damage.2）The dynamic changes of Calpain and Ubiquitin content in skeletal muscle after short-term repeated eccentric training was almost consistent with the dynamic changes of skele

关 键 词：离心运动 骨骼肌 超微结构 肌肉损伤 CALPAIN UBIQUITIN 鼠 动物实验 

分 类 号：G804.7[文化科学—运动人体科学]