氧钒离子作用下人血红蛋白构象变化的动态过程及2,3-二磷酸甘油酸的影响  

The Process of Conformational Change of Human Hemoglobin Induced by Vanadyl Ion Binding and the Effect of 2,3\|Diphosphoglycerate

作　　者：薛德平[1,2,3] 林华宽[1,2,3] 王夔 

机构地区：[1]南京大学化学化工学院 [2]南开大学化学化工学院 [3]北京医科大学无机药物及生物无机化学系

出　　处：《高等学校化学学报》1999年第2期167-171,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：用停流分光光度计检测在有或无ＤＰＧ结合时Ｈｂ与氧钒离子作用过程中自身荧光的变化，发现无ＤＰＧ的Ｈｂ自身荧光只是一个单调的荧光猝灭过程．ＤＰＧ结合于Ｈｂ后的荧光变化则先为荧光猝灭，转而缓慢增加．The process of the conformational change of human hemoglobin as the result of its interaction with vanadyl ions with and without DPG was determined by means of stopped flow measurement of the intrinsic fluorescence of Hb. The vanadyl binding causes monotonous quenching of the intrinsic fluorescence of DPG\|depleted Hb. It is noteworthy that the binding of DPG to Hb makes the process to be biphasic one. The first step is also featured by the quenching of intrinsic fluorescence. In the second step of DPG\|Hb system, the intrinsic fluorescence stops to decrease and turns to increase very slightly. The process was explained on the basis of vanadyl\|binding induced conformation change and the stabilizing effect of DPG and its hydrolytic product 3PGA to the tetrameric structure of Hb.

关 键 词：氧钒离子 血红蛋白 构象变化 生物效应 钒 

分 类 号：Q584.04[生物学—生物化学] Q513.04