水通道蛋白-1在结核性胸腔积液大鼠胸膜上的表达  

The expression of aquaporin-1 on the pleura of rat model with tuberculous pleural effusion

作　　者：杜宏春[1] 谢灿茂[1] 何桥[2] 李方知[2] 邓晓华[2] 

机构地区：[1]中山大学附属一院呼吸内科,广州510080 [2]广州市胸科医院病理科,广州510095

出　　处：《中国防痨杂志》2010年第6期301-305,F0003,共6页Chinese Journal of Antituberculosis

基　　金：广东省科技攻关项目(2006B36007019)

摘　　要：目的探讨胸膜上水通道蛋白-1(AQP-1)的表达与结核性胸腔积液大鼠胸液形成之间的关系。方法 48只健康Wistar大鼠随机分为结核性胸腔积液1d组,3d组,5d组和生理盐水对照组(每组各12只),实验组胸膜腔内注射标准人型结核分枝杆菌。应用免疫组织化学检测AQP-1在大鼠胸膜上的分布及蛋白质的表达。实时荧光定量RT-PCR及westernblot方法检测各组大鼠AQP-1在结核性胸腔积液形成前后胸膜上mRNA及蛋白表达的变化。结果大鼠胸腔内注射结核杆菌5d内均有双侧胸腔积液,胸腔积液量于第3d最多(4.4±1.2ml),结核性胸腔积液1d组,3d组和5d组脏壁层胸膜AQP-1mRNA和蛋白表达均高于生理盐水对照组,P均<0.05。结论结核性胸腔积液大鼠胸膜的AQP-1表达增多,AQP-1的增多可能与结核性胸腔积液的形成有关。Objective To investigate whether the expression of aquaporin-1（AQP-1） on the pleura is related with tuberculous pleural effusion in a rat model. Methods Forty-eight Wistar rats were randomly and equally divided into tuberculous pleural effusion （days 1,3,and 5） and control groups.The rats in tuberculous pleural effusion groups were injected into pleural cavities with 2 ml（9×10^6 CFU） of Mycobacterium tuberculosis strain H37Rv,and the rats in control group were injected with 2 ml of saline. The visceral and parietal pleural tissues were taken and processed for detecting the level of AQP-1 by real-time PCR （RT-PCR）,Western blot,and immunohistochemistry. Results The rats in tuberculous pleural effusion group（days 5） showed bilateral pleural effusions. The peak amount of pleural fluid occurred on day 3 （4.4±1.2 ml）. The level of AQP-1 mRNA and protein were significantly increased in both the parietal and visceral pleura from the rats in the tuberculous pleural effusion groups as compared with the control group （P〈0.05）. Conclusions The expression of AQP-1 was increased on the pleura of rats with tuberculosis. These results suggest AQP-1 is likely involved in the formation of tuberculous pleural effusions.

关 键 词：结核 胸膜 胸腔积液 水通道蛋白质-1 

分 类 号：R521.7[医药卫生—内科学]