多肽IS4溶液构象的进一步NMR研究  

Further NMR Investigation and Conformation\= Study of Polypeptide IS4

作　　者：涂光忠[1,2] 苗振伟[1,2] 张日清[1,2] 罗雪春[1,2] 蒋云[1,2] 赵南明[1,2] 

机构地区：[1]生物膜与生物膜工程国家重点实验室清华大学生物科学与技术系 [2]北京大学化学与分子工程学院

出　　处：《高等学校化学学报》1999年第2期264-267,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金攀登项目资助

摘　　要：利用二维核磁共振技术对多肽ＩＳ４中除Ｓｅｒ－１外的所有残基质子进行了指认．积分ＮＯＥＳＹ谱中的相关峰可得到距离约束．二面角的约束来自偶合常数３ＪＮＨα．由慢交换质子可得到氢键的约束．在进行距离几何程序计算时利用这些约束可得到一组构象，用能量最优化程序优化后的结果表明。Two\|dimensional \{\}\+1H NMR technique was used to determine the solution structure of IS4. The cross\|peaks volumes in NOESY spectra were used to generate proton\|proton distance constraints. These data, combined with hydrogen bond constraints and angle constraints, were used to obtain a set of 10 structures by program DIANA. These structures were refined by unrestrained energy minimization using standard minimization program. The results indicated that the conformation of IS4 in CF\-3CD\-2OD is of \|helix.

关 键 词：多肽IS4 NMR 构象 结构计算 IS4 

分 类 号：Q516.03[生物学—生物化学]