基体辅助激光解吸/电离质谱法对核酸分子的分析测定  

Determination of Nucleotide Molecules by Matrix assisted Laser Desorption/Ionization Mass Spectrometry

作　　者：邓慧敏[1] 黎军[1] 赖志辉 赵善楷 

机构地区：[1]中山大学测试中心

出　　处：《应用化学》1999年第1期95-97,共3页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金

摘　　要：基体辅助激光解吸／电离质谱（ＭＡＬＤＩＭＳ）法自８０年代末由Ｋａｒａｓ，Ｈｉｌｅｎｋａｍｐ报道以来，已获得极大的发展．近几年来，其应用范围迅速扩大，各种生物大分子如蛋白质，核酸（ＤＮＡ），多糖等都已能用ＭＡＬＤＩＭＳ法进行分子量测定．１９９３年，...Matrix assisted laser desorption/ionization time of flight mass spectrometry has been used to determine the molecular weights of DNA oligomers d(T) 6 and d(C) 10 . The results showed that 3 hydroxypicolinic acid was effective matrix in comparision with the mixture of 3 hydroxypicolinic acid and picolinic acid, 2,5 dihydroxybenzoic acid, α cyano 4 hydroxycinnamic acid, 2,5 dihydroxyacetophenone and 2,4,6 trihydroxyacetophenone. NH + 4 ion exchane resin and diammonium hydrogen citrate were used successfully to eliminate the influences of Na +, K +, Ca 2+ , Mg 2+ ions.

关 键 词：核酸 分子量 测定 MALDI MS 

分 类 号：Q520.3[生物学—生物化学]