依达拉奉对脑创伤大鼠学习记忆功能影响  

Effect of edaravone on learning-memory function following traumatic brain injury in rats

作　　者：赵雅宁[1] 高俊玲[1] 饶颖臻[1] 崔建忠 田艳霞[1] 张宇新[1] 

机构地区：[1]华北煤炭医学院河北省煤矿卫生与安全重点实验室,河北唐山063000 [2]河北省唐山市工人医院

出　　处：《中国公共卫生》2010年第6期753-754,共2页Chinese Journal of Public Health

基　　金：河北省自然科学基金(2009001247);河北省博士基金(06547008D-7);中国人事部留学归国基金(2007-17)

摘　　要：目的探讨依达拉奉(edaravone)对脑创伤的保护作用。方法 112只雄性SD大鼠随机分为3组:对照组、创伤组、依达拉奉干预组。Marmarou′s法建立弥漫性脑创伤模型。电镜观察海马区神经细胞形态变化;蛋白印迹(Western-Blot)法检测磷酸化细胞外信号调节激酶1/2(ERK1/2)表达;水迷宫法测试动物学习记忆功能。结果与对照组比较,磷酸化ERK1/2在伤后1～48h表达水平明显增高(P<0.05);学习记忆指标潜伏期明显延长(P<0.05)。依拉达奉干预组磷酸化ERK1/2表达水平在1,6,24与48h分别为(1.29±0.27),(3.48±1.49),(5.39±2.13)和(1.90±0.72),与创伤组比较明显下降(P<0.05);依拉达奉干预组学习记忆指标潜伏期分别为186.8,100.9,70.6,63.5和60.9s,明显短于创伤组(P<0.05)。结论依拉达奉可抑制脑创伤后ERK1/2活化,对脑创伤后学习记忆损害有改善作用。Objective To study the effect of edaravone on learning-memory function and its potential protective mechanism after traumatic brain injury（TBI）.Methods Male Sprague-Dawley rats were divided randomly into three groups：control,traumatic,and edaravone treatment group.TBI rat model was established according to the description of Marmarou′s diffused brain injury.Changes of neuron morphous were observed with electron microscope.The ERK1/2 phosphorylation expression was detected with Western Blot.The learning and memory function were detected with Morris water maze.Results Compared wih the control group,some neurons displayed severe histopathologic injury.The ERK1/2 phosphorylation expressions were significantly enhanced at 1hr（3.79±0.79）,6hr（6.60±1.19）,24hr（9.68±1.79）,and 48hr（3.34±0.82）.The mean of latency period in traumatic group（230.9±20.9s） was longer than that of in the control group（50.7±4.9s）.Compared to traumatic group,the neuronal histopathologic injury decreased,and the ERK1/2 phodphorylation expressions were decreased at 6hr（3.48±1.49）,24hr（5.39±2.03）,48hr（1.90±0.72）,（P0.05 for all）.The mean of latency period shortened（70.6±8.7,P0.05）.Conclusion Edaravine can dramatically improve learning and memory function after TBI.Edaravone may down-regulate ERK1/2 activation after TBI.

关 键 词：脑创伤 细胞外信号调节MAP激酶 依达拉奉(edaravone) 

分 类 号：R651[医药卫生—外科学]