大连沿海鼠尾藻野生种群遗传结构的ISSR分析  

Genetic structure of wild Sargassum thunbergii populations along Dalian coast:An ISSR analysis

作　　者：王萌[1,2] 李世国[1,2] 侯和胜[1,2] 王丽梅[3] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029 [2]辽宁省植物生物技术重点实验室,辽宁大连116029 [3]辽宁省海洋水产科学研究院,辽宁大连116023

出　　处：《生态学杂志》2010年第6期1181-1186,共6页Chinese Journal of Ecology

基　　金：辽宁省科学技术计划重大项目(2008203002);辽宁省海洋与渔业科研项目(200801);大连市科技兴海示范工程资助项目(2007BNC065)

摘　　要：为了解大连沿海鼠尾藻野生种群的遗传结构,采用ISSR分子标记技术对5个采自辽宁大连和1个采自山东蓬莱的野生鼠尾藻种群进行遗传多样性和亲缘关系分析。利用筛选出的14个ISSR引物共扩增得到160个位点,其中多态性位点145个,多态位点个数占90.62%。6个种群的多态位点百分率(PPB)分布为41.25%～64.38%,基因多样性指数(H)为0.2321～0.3464,而Shannon信息指数(I)则为0.1585～0.2333,大连沿海鼠尾藻种群的遗传多样性水平高于蓬莱种群。分子变异分析(AMOVA)结果表明:在总的遗传变异中,种群内部变异为5.66%,种群之间为64.34%。基因流Nm=0.7837,各种群间存在有限的基因交流。UPGMA聚类结果显示,大长山(DC)、董坨子(DT)和将军石种群(JJ)聚为一支,亲缘关系较近,再与石城岛(SC)和盐场种群(YC)聚类,最后与蓬莱种群(PL)聚类在一起。鼠尾藻的生殖方式以及生长环境的差别可能是其种群遗传分化的主要原因。In order to understand the genetic structure of wild Sargassum thunbergii populations along Dalian coast, an inter-simple sequence repeat （ISSR） analysis was made on the genetic diversity and genetic relationships of six geographic populations of S. thunbergii, including five populations DC, DT, JJ, SC and YC from Dalian coast and one population PL from Penglai. 160 ISSR loci tested, 145 （90.62%） were polymorphic with 14 different ISSR primers. The values of percentage of polymorphic bands （ PPB ）, Shannon＇ s index （ I）, and Nei＇ s gene diversity （H） were 41.25% -64. 38%, 0. 2321 -0. 3464, and 0. 1585 -0. 2333, respectively. Dalian populations had higher genetic diversity than Penglai population. The analysis of molecular variance （AMOVA） showed that in the total genetic variation, the variation within populations was 35.66%, and the variation among populations was 64.34%. The value of gene flow （Nm ） among the six populations was 0. 7837, suggesting a limited gene flow among the populations. The UPGMA tree based on Nei＇s genetic identity showed that population DC was clustered with populations DT and JJ, then with populations SC and YC, and finally with PL. All the results suggested that different reproductive mode and different growth environment might have critical roles in the genetic differentiation of S. thunbergii populations.

关 键 词：鼠尾藻 ISSR 遗传多样性 亲缘关系 

分 类 号：Q943[生物学—植物学]