莠去津慢性染毒对河蟹血淋巴细胞DNA的影响  

Impacts of atrazine chronic poisoning on hemolymph DNA of Eriocheir sinensis

作　　者：穆淑梅[1] 康现江[1] 张志甫[1] 郭明申[1] 李凤超[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《生态学杂志》2010年第6期1240-1244,共5页Chinese Journal of Ecology

基　　金：河北省自然科学基金资助项目(C2008000591)

摘　　要：利用不同浓度(0.01、0.1、1mg·L-1)的莠去津对河蟹进行慢性染毒处理90d,采用单细胞凝胶电泳技术检测其对河蟹血淋巴细胞DNA损伤的影响。结果表明,不同浓度的莠去津能引起河蟹血淋巴细胞的DNA损伤,且随着浓度的增加,TM值、TailDNA逐渐变大,尤以1mg·L-1浓度组最为显著。证明一定浓度的莠去津可以造成河蟹血淋巴细胞DNA链的断裂,破坏其DNA结构。Atrazine is one of the most widely used herbicides in China. In this paper, Erioeheir sinensis was treated with 0. 01, 0. 1, and 1 mg · L^-1 of atrazine for 90 days, and single-cell gel eleetrophoresis was used to study the impacts of atrazine chronic poisoning on the hemolymph DNA of E. sinensis. The fluorescence microscope observation and the＇CASP analysis of the images showed that all the three concentrations of atrazine could induced the DNA damage of E. sinensis hemolymph. With the increase of atrazine concentration, the TM value and the percentage of TailDNA increased gradually, and the impacts were most significant when the concentration of atrazine was 1 mg · L^-1 , suggesting that definite concentration atrazine could induce the broken of DNA chain, and destroy the DNA structure of E. sinensis hemolymph.

关 键 词：莠去津 河蟹 血淋巴细胞 DNA损伤 单细胞凝胶电泳 

分 类 号：X503.225[环境科学与工程—环境工程]