超声化学法制备蛋白质微球  

Sonochemical Synthesis of Proteinaceous Microspheres

作　　者：王晓明[1] 滕新荣[1] 

机构地区：[1]同济大学材料科学与工程学院,上海200092

出　　处：《化学进展》2010年第6期1086-1093,共8页Progress in Chemistry

基　　金：上海市纳米技术专项(No.0852nm05400);教育部留学回国人员科研启动基金(No.20090315)资助

摘　　要：采用高强度的超声波照射油/蛋白质水溶液的两相界面,可以得到载水不溶性液体的蛋白质微球。本文首先介绍了超声化学的背景知识,然后叙述了利用超声化学反应制备蛋白质微球的方法与成球机理,重点对蛋白质的交联与改性自组装做了较为详细的介绍。此外,还叙述了不同实验条件,如超声波照射时间、超声功率、探头位置、油水相体积分数等对蛋白质微球性能的影响,并对蛋白质微球的生物活性进行了探论。最后介绍了蛋白质微球的应用,如作为药物载体及在其他生物领域的应用等。这种超声化学反应方法为聚合物微球的制备提供了一个新的研究思路,在生物医药领域将有广泛的应用。Proteinaceous microspheres filled with water-insoluble liquids can be obtained by using highintensity ultrasound to irradiate the interface of two phases：oil /aqueous protein solution.The review starts with a short introduction to sonochemistry,and then the fabrication and synthesis mechanism of proteinaceous microspheres are described in detail.During the formation of proteinaceous microspheres,the shell of protein can be cross-linked or not.For those non cross-linked protein,modifications such as electrostatic layer by layer assembly could be used to make microspheres stabilized and functionalized.Experimental conditions such as sonication time,power amplitudes and the position of sonicator probe placed at the oil-water interface as well as the ratios of oil-water on the yields,size and size distribution of proteinaceous microspheres are described in detail.Biological activity of proteinaceous microspheres is also discussed in this review.Most results indicated that proteinaceous microspheres could still maintain their biologically active.Finally the potential applications of the proteinaceous microspheres are outlined,which vary from as drug carriers,magnetic contrast agents to fluorescence labeling carriers,etc.Such sonochemical method provides us a new,novel idea to preparation of polymer microspheres and will have prospective applications in biological filed,etc.

关 键 词：超声化学 蛋白质微球 油水界面 

分 类 号：O644.3[理学—物理化学] O629.7[理学—化学]