用CBMN法评价4-氯酚与富里酸对CHO细胞的毒性效应  

The Assessment of the Cytotoxicity Effects of 4 CP and FA on the CHO Cell by Cytokinesis Block Micronucleus Assay *

作　　者：李素文[1,2,3] 刘艳红[1,2,3] 王文华 柴文[1,2,3] 郁志勇 

机构地区：[1]北京师范大学生物学系 [2]中国科学院生态环境研究中心 [3]北京市第九水厂

出　　处：《环境科学》1999年第2期61-64,共4页Environmental Science

基　　金：国家自然科学基金;北京市自然科学基金

摘　　要：用ＣＨＯ细胞和ＣＢＭＮ法对４－ＣＰ（４－氯酚）及其ＦＡ（富里酸）协同作用的细胞毒性效应进行了评价，选用的细胞学毒性参数为总微核率（ＭＮ）、含微核的双核细胞率（ＢＮＭＮ）、核分裂指数（ＮＤＩ）和胞质分裂阻断增殖指数（ＣＢＰＩ）．结果表明：ＦＡ（０．６６ｍｇ·Ｌ－１）单独处理与阴性对照组比较不引起显著性差异；４－ＣＰ和ＦＡ（０．６６ｍｇ·Ｌ－１）联合处理与４－ＣＰ各浓度分别处理相比有显著性的差异；对于ＢＮＭＮ和ＮＤＩ，Ｐ＜０．０１；对于ＭＮ和ＣＢＰＩ，Ｐ＜０．０５，协同作用使细胞毒性效应增强．在２种处理下，４－ＣＰ的剂量与细胞毒性效应之间都存在显著性相关，当４－ＣＰ剂量大于１５ｍｇ·Ｌ－１时，除参数ＭＮ外，其余各细胞毒性参数与阴性对照组相比都有显著或非常显著的差异．该工作提示：由于氯酚类物质本身具有较强的细胞毒性效应以及与水体中ＦＡ协同作用后又能增强细胞毒性，所以应严格控制氯酚类物质对水体的污染．By means of the cytokinesis block micronucleus(CBMN) assay for the CHO cell,the synergism effect of 4 CP and FA on the cytotoxicity parameters were evaluated.These cytotoxicity parameters include the frequencies of micronuclei (MN),the frequencies of micronucleated binucleated cells (BNMN),the nuclear division index (NDI),and the cytokinesis block proliferation index (CBPI).The results indicated that the cytotoxicity effects with FA(0 66mg·L 1 ) alone is not statistically significantly different compared with negative control.The effects of combination of 4 CP at the doses of 5,10,15 and 20mg·L 1 respectively and FA(0 66mg·L 1 ) showed quite significant differences in MN,BNMN ( P <0 01),NDI ( P <0 01),MN( P <0 05) and CBPI( P <0 05) compared with that of 4 CP at the corresponding four doses respectively.For both treatments (4 CP with FA and 4 CP alone),4 CP at the doses more than 15mg·L 1 showed a significant differences in MN,BNMN,NDI and CBPI compared with negative control.Also for both treatments the correlativity between the dose of 4 CP and MN,BNMN,NDI and CBPI respectively were statistically significant.This work indicated that it is necessary to control strictly the pollution of chlorophenol in aquatic system due to their synergism effects on cytotoxicity.

关 键 词：细胞毒性参数 富里酸 4-氯酚 CBMN法 水体污染 

分 类 号：X520.32[环境科学与工程—环境工程]