海岛棉DELLA蛋白编码基因GbGAI的克隆与功能初步分析  

Cloning and Preliminary Functional Analysis ofGbGAI1 Gene whichEnconding DELLA Protein fromGossypium barbadense L.

作　　者：曲廷云[1] 王拴锁[2] 彭明[1,3] 崔百明[1] 郑银英[1] 

机构地区：[1]石河子大学农业生物技术重点实验室∕石河子大学生命科学院,新疆石河子832003 [2]中国科学院遗传与发育生物学研究所植物细胞与染色体工程国家重点实验室,北京100101 [3]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《中国农学通报》2010年第13期30-35,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金(307600991);兵团博士资金(2007JC08);石河子大学博士基金(RCZ200620)

摘　　要：赤霉素在棉花纤维发育过程中起着重要作用。DELLA蛋白是赤霉素信号传导通路中的一类重要负调节因子。通过同源克隆的方法,克隆了新疆特有棉花栽培种海岛棉中DELLA蛋白的同源基因GbGAI。序列比对分析表明GbGAI编码的氨基酸序列具有DELLA蛋白的典型结构域。构建其过量表达载体,通过农杆菌侵染花序的方法转入野生型拟南芥中,观察表型。转基因株系表现出晚花,株高变矮等赤霉素不敏感突变体的表型。Gibberllins（GAs） plays important roles in cotton fiber development. DELLA proteins, a negative regulator in the signaling pathway of GAs. The gene GbGAI enconding the DELLA protein in Gossypium hirsutum L. was isolated and cloned. The typical domains of DELLA proteins were also found in the GbGAI proteins. Over-express lines of GbGAI were got by dipping the 35S： GbGAI vector into the wild type Arabidopsis columbia, and these lines showed phynotypes such like late flowering, severely dwarf et al. Which exactly were same to the GAs deficient or insensitive mutant.

关 键 词：海岛棉 赤霉素 DELLA蛋白 拟南芥 转基因植株 

分 类 号：Q751[生物学—分子生物学]