肇东苜蓿和Pleven6苜蓿离体再生体系的建立  

Alfalfa Establishment of in vitro Regeneration System of Zhaodong Alfalfa and Pleven6

作　　者：麻晓春[1] 张月学[2] 张海玲[2] 尚晨[2] 李佶恺[2] 徐香玲[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025 [2]黑龙江省农业科学院草业研究所,哈尔滨150086

出　　处：《中国农学通报》2010年第13期47-52,共6页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划"小宗作物空间环境诱变育种关键技术研究与示范"(2008BAD97B07);黑龙江省农科院创新工程"牧草育种方法创新研究"(200705)

摘　　要：以肇东紫花苜蓿和Pleven6紫花苜蓿的子叶、下胚轴为外植体,建立离体再生体系。结果如下:最佳愈伤诱导培养基:肇东苜蓿为MS+2.0mg/L2,4-D+0.5mg/LKT+3%蔗糖;Pleven6为MS+1.5mg/L2,4-D+0.5mg/LKT+3%蔗糖;分化培养基:MS+2.0mg/L6-BA+0.5mg/LNAA+3%蔗糖;分化继代培养基:MS+0.3mg/L6-BA+0.1mg/LNAA+0.3mg/LKT+3%蔗糖;生根培养基:1/2MS+0.5mg/LNAA+1.5%蔗糖。2个品种的子叶均较下胚轴分化时间短、分化效率高,肇东苜蓿诱导率和分化率明显高于pleven6苜蓿。The cotyledons and hypocotyls explants from Zhaodong alfalfa and Pleven6 alfalfa, the establishment of in vitro regeneration system. The results are as follows： the best callus induction medium of Zhaodong alfalfa was MS＋2.0 mg/L2,4-D＋0.5 mg/ LKT＋3% sucrose and of Pleven6 was MS＋ 1.5 mg/L2,4-D ＋ 0.5 mg/LKT ＋ 3% sucrose; Differentiation medium： MS ＋ 2.0 mg/L6-BA ＋ 0.5 mg/LNAA ＋ 3% sucrose; Differentiation subculture medium： MS ＋ 0.3 mg/L6-BA ＋ 0.1 mg/LNAA ＋ 0.3 mg/LKT ＋ 3% sucrose; Induced rooting medium： 1/2MS＋0.5 mg/LNAA＋ 1.5% sucrose. At the same level of the experiment, induction rate and differentiation rate of Zhaodong alfalfa was significantly higher than pleven6 alfalfa, and both cotyledons used shorter differentiation time and higher differentiation rate than hypocotyls.

关 键 词：紫花苜蓿 离体再生体系 子叶 下胚轴 

分 类 号：S336[农业科学—作物遗传育种]