甜瓜SSR引物在西葫芦中的通用性  

Transferability of SSRs from Sweet Melon to Summer Squash

作　　者：刘栓桃[1] 董艳敏[1] 赵智中[1] 卢亚楠[1] 卢金东[1] 何启伟[1] 

机构地区：[1]山东省农业科学院蔬菜研究所,山东省设施蔬菜生物学重点实验室,国家蔬菜改良中心山东分中心,济南250100

出　　处：《中国农学通报》2010年第13期294-297,共4页Chinese Agricultural Science Bulletin

基　　金：山东省自然科学基金项目"西葫芦SSR引物开发及分子标记连锁图谱构建"(Q2006D11);山东省农业科学院创新基金项目"大白菜;西葫芦种质创新与分子标记研究及高效育种技术体系构建"(2006YCX014);山东省农业科学院青年基金项目"西葫芦SSR分子标记做图F2代及双单倍体(DH)群体构建与SSR引物的开发"(2005YQ003)

摘　　要：为了筛选更多的SSR标记用于西葫芦种质资源整理、评价和遗传研究,笔者首次利用西葫芦近缘物种甜瓜的SSR标记对中国现有西葫芦主要资源进行了多态性检测。结果表明,在所选的甜瓜25对SSR引物中有6对可在西葫芦基因组中有效扩增,占所选引物的24%。多态性分析发现,6对引物中有4对可以在34份西葫芦种质中检测到多态性,等位变异数从3～6个不等,平均为4个。UPGMA聚类分析表明,来自市场主栽F1的自交纯化材料和双单倍体材料之间多样性较差,说明现有栽培品种基因谱较窄;而从国家蔬菜种质资源库引进的材料多样性比较丰富,对这些材料的整理和重新评价有助于发掘新的基因源,可以为西葫芦杂交育种提供新材料。In order to screen more SSRs for summer squash （Cucurbita pepo L.） germplasm evaluation and genetic research, SSR primer pairs from sweet melon （Cucumis melo L.） were first used in polymorphism detection of summer squash. The results showed that approximately 24% of selected SSRs from Cucumis melo generated repeatable and clear products in summer squash. Of the transferable SSR markers, 4 primer pairs generated polymorphic products in 34 summer squash genotypes with a total of 16 alleles. Based on the SSR fingerprints, a relative low level of polymorphism was detected within the materials originated from F1 hybrid commonly seen in market while a high level of polymorphism was detected in those introduced from National Vegetable Ggermplasms Resource Center （NVGRC）. The results indicated that the cultivated groups commonly seen in market had a close relationship among each other, while those introduced from NVGRC were genetically diversified.

关 键 词：西葫芦 甜瓜 SSR 通用性 

分 类 号：S65[农业科学—果树学]