糖末宁颗粒剂对糖尿病周围神经病变大鼠坐骨神经NGF、IGF-1表达的影响  

Influences of Tangmoning Granules on sciatic expressions of NGF and IGF-1 proteins in rats with diabetic peripheral neuropathy

作　　者：王巍[1] 于世家[2] 于彩娜[3] 

机构地区：[1]中国人民解放军第202医院,辽宁110003 [2]辽宁中医药大学附属医院 [3]辽宁中医药大学

出　　处：《北京中医药大学学报》2010年第5期346-349,共4页Journal of Beijing University of Traditional Chinese Medicine

摘　　要：目的探讨糖末宁颗粒剂对糖尿病周围神经病变(DPN)、大鼠坐骨神经神经生长因子(NGF)、胰岛素样生长因子-1(IGF-1)蛋白表达的影响。方法将36只Wistar大鼠随机分为正常组、模型组、糖末宁组,采用2%链脲佐霉素(STZ)53 mg/kg诱导糖尿病大鼠模型。实验期间糖尿病大鼠每日灌服糖末宁混悬液(2.3 g/kg),实验结束取材后以透射电镜观察大鼠坐骨神经超微结构,应用蛋白印迹法(Western blot)及免疫组化技术检测各组大鼠坐骨神经NGF、IGF-1蛋白表达的情况。结果模型组大鼠坐骨神经NGF、IGF-1表达较正常组偏低,糖末宁组大鼠上述蛋白表达强于模型组,但较正常组仍降低。电镜结果显示,糖末宁颗粒剂可减轻大鼠坐骨神经的病理改变。结论糖末宁颗粒剂对DPN大鼠坐骨神经有一定的保护作用,其机制可能与上调NGF、IGF-1蛋白表达有关。Objective To discuss the influences of Tangmoning Granules on the expressions of sciatic nerve growth factor （NGF） and insulin-like growth factor-1 （IGF-1） proteins in rats with diabetic peripheral neuropathy （DPN）. Methods Wistar rats （n = 36） were randomly divided into the normal group, model group and Tangmoning Granules group. The rat model of diabetes was established by the injection of 2% streptozotocin （STZ, 53 mg/kg）. The diabetic rats were given orally the suspension of Tangmoning Granules （2.3 g/kg） every day during test. The sciatic uhrastructure was observed by using transmission electron microscope （TEM）, and the expressions of sciatic NGF and IGF-1 proteins were detected by applying Western blot method and immunohistochemistry in all groups. Results The expressions of sciatic NGF and IGF-1 proteins were lower in the model group than those in the normal group, and those in the Tangmoning Granules group was higher than those in the model group but lower than those in the normal group. The result of TEM showed that Tangmoning Granules relieved the pathological changes of rat sciatic nerve. Conclusion Tangmoning Granules has some protective effect on sciatic nerve in diabetic rats. The mechanism may be related to the up-regulation of the expressions of NGF and IGF-1 proteins.

关 键 词：糖末宁颗粒剂 糖尿病周围神经病变 生长因子 胰岛素样生长因子-1 大鼠 

分 类 号：R285.5[医药卫生—中药学]