辽宁省东港市三带喙库蚊对常用杀虫剂的抗药性  

Resistance of Culex tritaeniorhynchus in Donggang city,Liaoning province to commonly used insecticides

作　　者：靳建超[1,2] 孟凤霞[2] 苏建亚[1] 刘起勇[2,3] 丁俊[4] 王树诚[4] 陈哲 褚发军 

机构地区：[1]南京农业大学植物保护学院农药科学系农业部作物病虫害监测与防控重点开放实验室,南京210095 [2]中国疾病预防控制中心传染病预防控制所媒介生物控制室,北京102206 [3]传染病预防控制国家重点实验室,北京102206 [4]辽宁省疾病预防控制中心 [5]东港市疾病预防控制中心

出　　处：《中国媒介生物学及控制杂志》2010年第3期188-191,共4页Chinese Journal of Vector Biology and Control

基　　金：国家科技重大专项课题(2008ZX10004-010)~~

摘　　要：目的研究辽宁省东港市三带喙库蚊幼虫和成蚊对常用杀虫剂的敏感性,了解其对常用杀虫剂的抗药性水平,探讨我国三带喙库蚊幼虫和成蚊间的抗药性关系,为该蚊的化学控制提供理论依据。方法在三带喙库蚊的发生高峰期,大量采集野外三带喙库蚊成蚊,用小白鼠供血获得子一代为试虫。用WHO推荐的浸渍法、药膜接触法,分别测定三带喙库蚊幼虫和成虫对常用杀虫剂的敏感性。获得幼虫对常用杀虫剂的LC50及其95%置信限(95%CI)、成蚊的击倒率和死亡率,比较其抗药性程度。结果三带喙库蚊幼虫对溴氰菊酯、高效氯氰菊酯和氯菊酯的LC50及其95%CI分别为0.002098(0.001575～0.002866)、0.01380(0.01143～0.01664)和0.01651(0.01400～0.01949)mg/L,敌敌畏、双硫磷和杀螟硫磷LC50及其95%CI分别为25.3479(21.4949～28.3828)、48.3763(31.7172～92.4088)和0.1685(0.1085～0.2679)mg/L,仲丁威的LC50及其95%CI为0.2874(0.1803～0.4268)mg/L。与已发表文献的敏感品系比较,三带喙库蚊幼虫对溴氰菊酯、高效氯氰菊酯和氯菊酯相对抗性倍数分别为5.3、2.3和3.3倍,对敌敌畏、双硫磷和杀螟硫磷的相对抗性倍数分别为396.1、40313.6和34.0倍;三带喙库蚊成虫对0.05%溴氰菊酯(接触1h)、0.25%氯菊酯(3h)、1%杀螟硫磷(1h)24h后的死亡率分别为15.9%、27.7%和8.2%。结论辽宁省东港市的三带喙库蚊幼虫对有机磷产生了极高抗药性,对拟除虫菊酯处于低抗水平,但是成虫对溴氰菊酯、氯菊酯和杀螟硫磷具有较高的抗药性,针对拟除虫菊酯三带喙库蚊幼虫和成蚊的抗药性表现不同。Objective The susceptibility of the larvae and adult mosquitoes of Culex tritaeniorhynchus in Donggang city, Liaoning province to commonly used insecticides was evaluated to determine their insecticidal resistance and identify the relationship between the resistance levels of the larvae and adult mosquitoes, providing the basis for chemical control of the mosquitoes. Methods A large number of adult Cx. tritaeniorhynchus were collected outdoors in the peak seasons to obtain the first filial generation, fed with the blood of mice, for testing. The impregnation method and the contact exposure method, as recommended by the WHO, were employed to determine the susceptibility of the larvae and adult mosquitoes of Cx. tritaeniorhynchus to commonly used insecticides, respectively, for calculating the LC50 and the corresponding 95% confidence limits （95%CI） for the larvae, and the knockdown rate and mortality of the adult mosquitoes for comparison of their resistance levels to commonly used insecticides. Results The LC50 and the corresponding 95%CI of deltamethrin, beta-cypermethrin, and permethrin for Cx. tritaeniorhynchus larvae were 0.002 098 （0.001 575- 0.002 866）, 0.013 80 （0.011 43-0.016 64） and 0.016 51 （0.014 00-0.019 49） mg/L, respectively; the LC50 and the corresponding 95%CI of dichlorvos, temephos and fenitrothion were 25.3479 （21.4949-28.3828）, 48.3763 （31.7172-92.4088） and 0.1685 （0.1085-0.2679） mg/L, respectively; the LC50 and the corresponding 95%CI of BPMC were 0.2874 （0.1803-0.4268） mg/L. Compared to the susceptible strains reported in previous literature, the larvae of Cx. tritaeniorhynchus had the resistance coefficients of 5.3, 2.3 and 3.3 to deltamethrin, beta-cypermethrin and permethrin, respectively, and 396.1, 40 313.6 and 34.0 to dichlorvos, temephos and fenitrothion, respectively; the mortality rates of the adult Cx. tritaeniorhynchus exposed to 0.05% deltamethrin （contact for 1 h）, 0.25% permethrin （3 h）, and 1% fenitrothion （1 h） beyond 24 h were 15.9

关 键 词：三带喙库蚊 拟除虫菊酯 有机磷 抗药性 

分 类 号：R384.1[医药卫生—医学寄生虫学] S481.4[医药卫生—基础医学]