苦参饮片质量标准研究  

Quality Standard of Sophora flavescens Decoction

作　　者：王惠娟[1] 杨国亮[2] 麻风华[3] 张清波[3] 

机构地区：[1]佳木斯大学附属第一医院药剂科,佳木斯市154002 [2]黑龙江绥化市药品检验所,绥化市152062 [3]黑龙江省药品检验所,哈尔滨市150001

出　　处：《中国药房》2010年第23期2151-2153,共3页China Pharmacy

基　　金：<中国药典>2010版(一部)标准研究课题(YZ-073)

摘　　要：目的:建立苦参饮片的质量标准。方法:采用薄层色谱(TLC)法对制剂进行定性鉴别,采用高效液相色谱(HPLC)法测定制剂中苦参碱和氧化苦参碱的含量。结果:TLC中斑点清晰、分离度好。苦参碱、氧化苦参碱的进样量分别在0.02494～0.64844μg(r=0.9999)、0.0734～1.9084μg(r=0.9999)范围内与各自峰面积积分值呈良好线性关系;二者平均回收率分别为102.9%、102.3%,RSD分别为6.9%、2.7%(n=6)。结论:所建标准可用于苦参饮片的质量控制。OBJECTIVE：To establish the quality standard of Sophora flavescens decoction.METHODS： The preparation was determined qualitatively using TLC,and the contents of matrine and oxymatrine were determined by HPLC.RESULTS： The TLC spots of S.flavescens decoction were clear and well-separated.The linear ranges of matrine and oxymatrine were 0.024 92～0.648 44 μg（r=0.999 9） and 0.073 4～1.908 4 μg（r=0.999 9）.The average recoveries were 102.9% and 102.3% and RSDs were 6.9% and 102.3%,respectively （n=6）.CONCLUSION： Establish standard can be used for the quality control of S.flavescens decoction.

关 键 词：苦参饮片 薄层色谱法 高效液相色谱法 苦参碱 氧化苦参碱 

分 类 号：R283.64[医药卫生—中药学] R927.1[医药卫生—中医学]