复方丹参片的HPLC指纹图谱研究  

Study on HPLC Fingerprint of Compound Danshen Tablets

作　　者：王颖[1] 

机构地区：[1]广西柳州市柳铁中心医院,柳州市545007

出　　处：《中国药房》2010年第23期2162-2163,共2页China Pharmacy

摘　　要：目的:应用高效液相色谱(HPLC)法建立复方丹参片的指纹图谱。方法:色谱柱为Agilent Eclipse XDB-C18(150mm×4.6mm,5μm),流动相为乙腈-0.04%磷酸水(梯度洗脱),检测波长为272nm,流速为1.0mL·min-1,柱温为30℃。结果:所建指纹图谱特征性强、重复性好。共标定出8个指纹特征峰,其中5、6、7、8号特征峰来源于组方中的丹参,1、2、3、4号特征峰来源于组方中的三七。结论:复方丹参片药效部分特征峰主要源于丹参。本试验可为复方丹参片有效成分的追踪分离及中成药的二次开发奠定基础。OBJECTIVE： To establish HPLC fingerprint of Compound danshen tablets.METHODS： The separation was performed on Agilent Eclipse XDB-C18（150 mm×4.6 mm,5μm） column with mobile phase consisted of acetonitrile-0.04% phosphate （gradient elution） at flow rate of 1.0 mL·min-1.Detection wavelength was set at 272 nm and column temperature was 30℃.RESULTS： Established HPLC fingerprint was distinct and reproducible.There were 8 characteristic peaks among which No.5 characteristic peaks,No.6,No.7,No.8 came from HPLC fingerprint of Salvia miltiorrhiza and No.1 characteristic peaks,No.2,No.3,No.4 came from HPLC fingerprint of Panax notoginseng.CONCLUSION： The characteristic peaks of effective parts of Compound danshen tablet mainly come from S.miltiorrhiza.The study can be used for tracing and isolation of effective part of Compound danshen tablet and establishes basis for the further development of Chinese patent drugs.

关 键 词：复方丹参片 高效液相色谱法 指纹图谱 化学成分归属 

分 类 号：R283.64[医药卫生—中药学] R927[医药卫生—中医学]