GC法测定补骨脂酊中补骨脂素和异补骨脂素的含量  

Content Determination of Psoralen and Isopsoralen in Buguzhi Tincture by GC

作　　者：马虹英[1] 

机构地区：[1]中南大学湘雅医院药剂科,长沙市410008

出　　处：《中国药房》2010年第23期2170-2171,共2页China Pharmacy

摘　　要：目的:建立以气相色谱法测定补骨脂酊中补骨脂素和异补骨脂素含量的方法。方法:色谱柱为HP-1弹性石英毛细管(30m×0.53mm,1μm),载气为高纯N2,检测器为FID,进样口温度为260℃,检测器温度为280℃,内标为苯海拉明。结果:补骨脂素和异补骨脂素的检测浓度分别在4.82～48.22mg·L-1、5.09～50.9mg·L-1范围内同各自峰面积与内标峰面积比值呈良好的线性关系(r均为0.9999);二者回收率分别为103.11%和100.98%,RSD分别为2.20%和3.70%(n均为6)。结论:本法操作简便、快速,结果准确、可靠,可用于补骨脂酊的质量控制。OBJECTIVE： To establish the GC method for content determination of psoralen and isopsoralen in Buguzhi tincture.METHODS： The determination was performed on HP-1（30 m×0.53 mm,1 μm） column with nitrogen as carrier gas and FID as detector,the inlet temperature was 260 ℃.The injector temperature was kept at 280 ℃ with diphenhydramine as internal standard.RESULTS：The linear range of psoralen were 4.82～48.22 mg·L-1（r=0.999 9） with an average recovery 103.11% （RSD=2.20%,n=6）.The linear range of isopsoralen were 5.09～50.9 mg·L-1（r=0.999 9） with an average recovery of 100.98% （RSD=3.70%,n=6）.CONCLUSION： This method is simple,rapid,accurate and reliable for the quality control of Buguzhi tincture.

关 键 词：补骨脂酊 补骨脂素 异补骨脂素 气相色谱法 含量 

分 类 号：R283.6[医药卫生—中药学] R927.2[医药卫生—中医学]