超声破坏促黄体生成激素释放激素靶向微泡联合紫杉醇对卵巢癌OVCAR3细胞株的影响  

Ultrasound destruction LHRH targeted microbubbles combined paclitaxel on ovarian cancer proliferation and apoptosis and intracellular drug concentration

作　　者：贺娟[1,2] 孙江川[3] 常淑芳[3] 李攀[4] 王志刚[4] 

机构地区：[1]成都市第九人民医院妇产科 [2]重庆医科大学附二院妇产科,硕士四川成都610010 [3]重庆医科大学附属第二医院妇产科,重庆400010 [4]重庆医科大学超声影像研究所,重庆400010

出　　处：《中国实用妇科与产科杂志》2010年第6期437-439,共3页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：国家863项目(2006AA02Z4F0);自然科学基金(30801228);重庆市自然科学基金(CSTC,2008BB5405)

摘　　要：目的探讨超声破坏卵巢癌靶向超声造影剂(ovarian cancer targeted ultrasound contrast agent,OCTUCA)即黏附促黄体生成激素释放激素(luteinizing hormone releasing hormone,LHRH)的脂质体微泡(LM)联合紫杉醇对人卵巢癌OVCAR3细胞株增殖、凋亡及细胞内药物浓度的影响。方法在体外培养OVCAR3细胞株,经MTT比色法检测紫杉醇对OVCAR3体外治疗的有效浓度,然后分别用OCTUCA、紫杉醇、紫杉醇联合超声、超声破坏脂质体微泡联合紫杉醇及超声破坏OCTUCA联合紫杉醇进行处理,MTT检测细胞增殖抑制率、流式细胞技术(FCM)检测细胞凋亡率、高效液相色谱(HPLC)法检测细胞内紫杉醇浓度。结果紫杉醇对OVCAR3体外治疗的有效浓度是10-6mol/L,超声破坏OCTUCA联合紫杉醇组细胞增殖抑制率及凋亡率均明显高于OCTUCA、紫杉醇、紫杉醇联合超声、超声破坏脂质体微泡联合紫杉处理组(P均<0.05),超声破坏OTUCA联合紫杉醇组细胞内药物浓度明显高于紫杉醇、紫杉醇联合超声、超声破坏脂质体微泡联合紫杉醇处理组(P<0.01)。结论超声破坏OCTUCA联合紫杉醇能使OVCAR3细胞内紫杉醇药物浓度增加,抑制其增殖,并诱导其凋亡。Objective Ultrasound destruct ovarian cancer targeted ultrasound contrast agent （OCTUCA）, another name is liposome microbubbles （LM） adhere with LHRH, to study the effect of which synergia paclitaxel treatment on ovarian cancer cell line OVCAR3 inducing change of proliferation, apoptosis and intracellular drug concentration. Method Culturing OVCAR3 cell line in vitro, to determine the appropriate concentration of paclitaxel treatment on OVCAR3 in vitro by MTT assay. Then using OCTUCA, paclitaxel, paclitaxel synergia ultrasound, ultrasound destruction LM synergia paclitaxel, ultrasound destruction OCTUCA synergia paclitaxel treat OVCAR3, determining the rate of cell proliferation by MTT assay, the rate of cell apoptosis by flow cytometry （FCM） assay, and the intracellular drug concentration by high performance liquid chromatogram （ HPLC ） assay. Result The appropriate concentration of paclitaxel treatment on OVCAR3 in vitro is 10^-6mol/L. The proliferation inhibition and apoptosis ratio of ultrasound destruction OCTUCA synergia paclitaxel were obviously higher than the group of OCTUCA, paclitaxel, paclitaxel synergia ultrasound, ultrasound destruction LM synergia paelitaxel （ P 〈 0.05 ）. The intracellular drug concentration of ultrasound destruction OCTUCA synergia paclitaxel was obviously higher than the group of paclitaxel, paclitaxel synergia ultrasound, ultrasound destruction LM synergia paclitaxel（ P 〈 0.01 ）. Conclusion Ultrasound destruction OCTUCA synergia paclitaxel treat OVCAR3 can cause intraeellular drug concentration accrescence, proliferation inhibition and induce apoptosis.

关 键 词：卵巢癌 靶向超声造影剂 促黄体生成激素释放激素 OVCAR3 

分 类 号：R71[医药卫生—妇产科学]