催化动力学光度法测定冷蒿中槲皮素  

Determination of Quercetin in Artemisiafrigida by Catalytic Kinetic Spectrophotometry

作　　者：刘雅静[1] 高扬[1] 李英杰[1] 马莹莹[1] 张力[1] 包玉敏[1] 

机构地区：[1]内蒙古民族大学化学化工学院,内蒙古通辽028043

出　　处：《内蒙古民族大学学报（自然科学版）》2010年第3期266-267,共2页Journal of Inner Mongolia Minzu University：Natural Sciences

基　　金：内蒙古自治区自然科学基金资助项目(200607010209);内蒙古高等学校自然科学基金资助项目(NJZY07138)

摘　　要：基于稀H_2SO_4介质中,槲皮素对KBrO_3氧化甲基橙褪色反应的明显催化作用,建立了测定槲皮素的动力学光度法.当甲基红、硫酸、KBrO_3的用量分别为1.00mL、0.30mL、0.25 mL,反应温度70℃,反应时间5min时,测定槲皮素的线性范围为0.00～0.05 mg/L,检出限为0.002 mg/L.利用此法测定了冷蒿中槲皮素含量,平均含量为0.2439×10^(-4)mg/g(n=4),加标回收率介于91.8%～96.9%,结果满意.A catalytic kinetic spectrophotometric method for the determination of quercetin was proposed.The method was based on a discoloring reaction of methyl red by quercetin as catalyst in the dilute sulfuric acid.The optimal reagent amount was 1.00 mL methyl red,0.30 mL H2SO4 and 0.25 mL KBrO3,respectively.The reaction temperature was 70℃ ,and reaction time was 5 min.The calibration curve was linear up to 0.05mg/L.The detection limit of the method was 0.002mg/L.The method was successfully applied to the determination of quercetin in the Artemisiafrigida. The content of Quercetin =0.2439 × 10^-4mg/g（n=4）,and the recovery 91.8% - 96.9 %.

关 键 词：冷蒿 槲皮素 甲基红 动力学光度法 

分 类 号：O657.3[理学—分析化学]