人磷酯酰蛋白聚糖3N端融合蛋白多克隆抗体制备及鉴定  

Preparation and characterization of polyclonal antibody against human glypican3 N-terminal protein

作　　者：江枫[1] 肖明兵[1] 赵敏星[1] 倪润洲[1] 周嘉伟[2] 

机构地区：[1]南通大学附属医院消化病研究室,南通226001 [2]中国科学院上海神经科学研究所

出　　处：《南通大学学报（医学版）》2010年第1期21-23,共3页Journal of Nantong University(Medical sciences)

基　　金：南通市社会发展计划2007指导项目(S7943)

摘　　要：目的:制备人磷酯酰蛋白聚糖3(Glypican3,GPC3)N端蛋白的多克隆抗体。方法:在大肠埃希菌Rosetta中诱导表达磷酯酰蛋白聚糖3N端融合蛋白,从SDS-PAGE中切胶纯化,免疫新西兰兔,制备多克隆抗体。用辛酸-硫酸铵分步沉淀法初步纯化抗血清,并用ELISA、Westen-blot检测抗体的灵敏度和特异性。结果:成功制备了抗磷酯酰蛋白聚糖3N端的多克隆抗体,抗体滴度为1:64000,经Western blot检测特异性良好。结论:成功制备了抗人磷酯酰蛋白聚糖3N端融合蛋白多克隆抗体。Objective：To prepare rabbit anti-human Glypican3 N-terminal protein polyclonal antibody.Methods：The Glypican3 N-terminal protein was expressed in E.coli Rosetta.The rabbits were immunized by purified protein to produce polyclonal antibody.The anti-sera were identified with ELISA and Western blot analysis.Results：The polyclonal antibody against Glypican3 N-terminal protein was successfully prepared and the titer of antisera was 1：64 000.Conclusion：The polyclonal antibody against human Glypican3 N-terminal protein was successfully prepared.

关 键 词：磷酯酰蛋白聚糖3 融合蛋白 多克隆抗体 原发性肝细胞癌 

分 类 号：Q78[生物学—分子生物学]