大鼠烫伤创面愈合过程中MMP-2和TIMP-1表达的相关性  

Correlation between expression of MMP-2 and TIMP-1 in the process of scald wound repairing in rats

作　　者：严志新[1] 王金龙[1] 陈淼[2] 魏中华[2] 陈建华[2] 

机构地区：[1]江苏省镇江市第一人民医院整形烧伤科,镇江212002 [2]江苏省镇江市第一人民医院病理科,镇江212002

出　　处：《南通大学学报（医学版）》2010年第2期86-88,F0002,共4页Journal of Nantong University(Medical sciences)

基　　金：镇江市卫生局科研资助项目(2003);镇江市重点医学人才基金资助项目

摘　　要：目的:探讨烫伤创面愈合过程中MMP-2和TIMP-1表达的相关性。方法:在8只大鼠背部制成20%TBSA深Ⅱ度烫伤模型,烫伤后分别在24小时,4,7,14,20,29,35,62天时切取每只大鼠背部创面标本,采用免疫组化方法检测标本中MMP-2及TIMP-1的表达情况;在实验中取2只正常大鼠皮肤标本,同样检测标本中MMP-2及TIMP-1的表达情况。结果:MMP-2和TIMP-1在正常大鼠皮肤中几乎无表达;在大鼠烫伤后不同时期表达强度不同,24小时后开始表达,以后逐渐增强,大约到第20天达到高峰,持续约15天后减弱;到创面愈合后仍有表达。MMP-2的表达水平与TIMP-1的表达水平呈正相关。结论:MMP-2和TIMP-1的表达水平在创面愈合过程中有一定规律,与烫伤创面的愈合和瘢痕的形成可能有密切关系。Objective：To investigate the correlation between expression of MMP-2 and TIMP-1 in the process of scald wound repairing.Methods：Backs of eight big rats were scalded into 20% TBSA deep partial thickness burn.Biopsies from wound skins of each rat harvested at 24PBHS and 4,7,14,20,29,35,62PBDS.The expressions of MMP-2 and TIMP-1 in the samples were detected immunohistochemically.MMP-2 and TIMP-1 were also detected in the skin of another 2 normal rats as normal contro1.Result：MMP-2 and TIMP-1 were hardly expressed in the normal skins of rats.After rats were scalded,MMP2 and TIMP-1 had different expressions in the process of scald wound repairing.MMP-2 and TIMP-1 began to express at 24 PBHS,and enhanced gradually,and peaked on 20PBDS,and continued for 15 days then reduced;but MMP-2 and TIMP-1 were still expressed up to wound healing.There were correlations between the expression of MMP-2 and the expression of TIMP-1.Conclusion：The expression levels of MMP-2 and TIMP-1 had certain laws during wound repairing,which were closely related to wound repairing and the process of scar tissue growing.

关 键 词：烧伤 基质金属蛋白酶 组织金属蛋白酶抑制剂 

分 类 号：R644[医药卫生—外科学]