移植物浸润T淋巴细胞肿瘤坏死因子相关凋亡诱导配体及受体DR4和DR5表达与大鼠小肠移植急性排斥反应的相关性  

Expression of TRAIL- DR4/DR5 in graft jejunums infiltrating T cells of rats small bowel transplantation in acute rejection

作　　者：李峰[1] 

机构地区：[1]河南省濮阳市油田总医院普外科,457001

出　　处：《国际移植与血液净化杂志》2010年第3期30-32,共3页International Journal of Transplantation and Hemopurification

摘　　要：目的探讨浸润T淋巴细胞上的DR4、DR5表达同小肠移植急性排斥反应的关系。方法将2种近交系大鼠（SD、Wistar）54只按随机配对法分为A、B、c3组。A组大鼠为对照组（18只）行虚拟手术；B组（18只）大鼠行同系小肠移植；C组（18只）大鼠行不同品系小肠移植。各组大鼠于术后5d取移植肠样本分别做HE染色和免疫荧光双标记染色。采用免疫荧光染色、激光共聚焦技术测定各组标本浸润T淋巴细胞肿瘤坏死因相关凋亡诱导配体及DR4、DR5的表达情况。结果A组大鼠小肠黏膜正常，B组大鼠小肠表现为免疫耐受，C组大鼠表现为急性排斥反应。C组大鼠高T淋巴细胞表达肿瘤坏死因子相关凋亡诱导配体与A、B组大鼠比较差异有统计学意义（P〈0．01）；A、B组大鼠浸润T淋巴细胞DR4、DR5均呈高表达，C组大鼠呈低表达，C组大鼠与A、B组比较差异有统计学意义（P〈0．01）；A、B组大鼠比较差异无统计学意义（P〉0．05）。结论急性排斥反应的发生可能与浸润T淋巴细胞DR的低表达有关。减少浸润淋巴细胞DR表达的下调，或者上调DR将有助于控制急性排斥反应，诱导免疫耐受。Objective To discuss the relations about acute rejection and DR4, DR5 expression on T cells in small bowel transplantatien. Methods Two species of inbred line rats （SD, Wistar） which were total 54 were randomly pair - matching divided into 3 groups（A, B, C ）. Group A（ n = 18） conducted fictitious operation; Group B（ n = 18） conducted iso- series small bowel transplantation（SBT） ; Group C（ n = 18） conducted anise series SBT. Samples of grafts were taken from 5 days after SBT. The grafts were stained by HE and immunofluorescent staining. To measure the expression of TRAIL, DR4, DR.5 with laser scanning confoeal microscope technique of INF- T cells in these specimens. Results The mucous membrane of small intestine in group A were normal, immunotolerance in group B, and acute rejection were expressd in group C. The expression of TRAIL in group C was significantly higher than that of group A and B （P 〈 0.01 ）. High expression of DR4, DR5 on INF - T cells were found in group A and B, whereas lower expression in group C; and significant difference was found between group C and the other two groups（ P 〈 0.01） ,but no significant difference was found between group A and group B （ P 〉 0.05）. Conclusion Acute rejection may be caused by the down regulation of DR4, DR5 expression on INF - T cells. To make up - regulation or reduce the down regulation expression level of DR might accelerate the apoptesis of INF- T cells and restrain the occurance of actue rejection in graft jejunums .

关 键 词：T淋巴细胞 肿瘤坏死因子相关凋亡诱导配体 小肠移植 急性排斥反应 

分 类 号：R392[医药卫生—免疫学]