日本血吸虫DNA鸡尾酒疫苗免疫研究  

Protective immunity of cocktail DNA vaccines against Schistosoma japonicum

作　　者：胡媛[1] 徐馀信[1] 沈玉娟[1] 卢潍媛[1] 刘述先[1] 曹建平[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,世界卫生组织疟疾、血吸虫病和丝虫病合作中心,上海200025

出　　处：《国际医学寄生虫病杂志》2010年第3期129-131,共3页International JOurnal of Medical Parasitic Diseases

基　　金：国家自然科学基金（30872212,30771880）;国家高技术研究发展计划（国家863计划）（2006AA02Z444）;国家传染病重大专项（2009ZX10004-201,2008ZX10004-011）;上海市科委生物医药重点课题（064319026）

摘　　要：目的 研究日本血吸虫DNA鸡尾酒疫苗的免疫保护效果. 方法 制备日本血吸虫谷胱甘肽S-转移酶（glutathione S-transferase,GST）、硫氧还蛋白（thioredoxins, Trx）、次黄嘌呤鸟嘌呤磷酸核糖转移酶（hypoxanthine-guanine phosphoriboribosyl-transferase,HGPRT）DNA疫苗,免疫C57BL/6小鼠.C57BE/6小鼠随机分为感染对照组、空质粒对照组、pVAX1-GST免疫组、pVAX1-Trx免疫组、pVAX1-HGPRT免疫组及DNA鸡尾酒疫苗组,依次注射生理盐水,空质粒pVAX1,pVAX1-GST,pVAX1-Trx,pVAX1-HGPRT和由pVAX1-GST、pVAX1-Trx、pVAX1-HGPRT等3种质粒混合而成的DNA鸡尾酒疫苗.每组小鼠于0、2、4周经股四头肌注射免疫,每次间隔2周.末次免疫后2周,每只小鼠经腹部皮肤感染30±1条日本血吸虫尾蚴.感染后42 d剖杀小鼠,并计数各组小鼠血吸虫成虫数和肝虫卵数. 结果 pVAX1-GST、pVAX1-Trx、pVAX1-HGPRT和DNA鸡尾酒疫苗免疫组小鼠的减虫率依次为0、35.78%、47.89%和48.13%,肝减卵率依次为22.94%、34.14%、43.35%和26.77%. 结论 pVAX1-Trx、pVAX1-HGPRT及DNA鸡尾酒疫苗具有一定的抗日本血吸虫病作用,但3种疫苗联合使用并未发现有显著的协同作用.Objective To investigate the protective immune efficacy against Schistosoma japonicum infection induced by cocktail DNA vaccines. Methods The male C57BL/6 mice were randomly divided into 6 groups （infected control group, pVAX1 group, pVAX1-GST group, pVAX1-Trx group, pVAX1-HGPRT group, and cocktail DNA vaccine group）. The mice in each group were injected with saline, plasmid pVAX1, pVAX1-GST, pVAX1-Trx, pVAX1-HGPRT, and the cocktail vaccine （mixture of pVAX1-GST, pVAX1-Trx and pVAX1-HGPRT） respectively for three times at 0, 2 and 4 week with interval of two weeks. Two weeks after the last immunization, all mice were challenged with cercariae of S. japonicum. Forty-two days post-challenge infection, the mice were sacrificed. The numbers of recovered worms and eggs in liver were counted. Result The worm reduction rates in pVAX1-GST group, pVAX1-Trx group, pVAX1-HGPRT group and cocktail DNA vaccine group were 0, 35.78% , 47.89% and 48. 13% respectively, while the egg reduction rates were 22.94% ,34. 14% ,43. 35% and 26. 77% , respectively. Conclusion The pVAX1-Trx, pVAX1-HGPRT and cocktail DNA vaccine exhibited a certain level of protective efficacy against schistosomiasis, however there were no significant synergetic effects using the cocktail DNA vaccine.

关 键 词：日本血吸虫 谷胱甘肽S-转移酶 硫氧还蛋白 次黄嘌呤鸟嘌呤磷酸核糖转移酶 鸡尾酒疫苗 

分 类 号：R383.24[医药卫生—医学寄生虫学]