低强度脉冲超声波防止废用性骨质疏松症  

Effects of Low Intensity Pulsed Ultrasound on Prevention of Disused Osteoporosis

作　　者：刘沐青[1] 郭霞[2] 王晓云[2] 

机构地区：[1]清华大学附属玉泉医院手外科,北京100049 [2]香港理工大学康复治疗科学系,香港999077

出　　处：《科技导报》2010年第11期43-46,共4页Science & Technology Review

摘　　要：肢体长期制动导致的骨质疏松症在临床中较为常见。本实验应用低强度脉冲超声波(LIPU)治疗大鼠废用性骨质疏松,试图探讨LIPU在防止废用性骨质丢失方面的作用及其作用机理。将40只Sprague-Dawley大鼠随机分为4组,每组10只。髌腱切除组(2组):切除左侧髌骨和5mm髌韧带,其中一组接受LIPU治疗,一组无治疗;神经切除组(2组):切除左侧坐骨神经1cm,其中一组接受LIPU治疗,另一组无治疗。超声波治疗组于左侧胫骨近端接受低强度脉冲超声波治疗。所有动物于4周后安乐死,取材。对双侧大鼠胫骨进行骨定量CT(pQCT)检查,测量平均骨密度(aBMD),以及骨小梁面积、周长,计算骨小梁体积(BV/TV,%)、宽度(Tb.Wi,μm)、数量(Tb.N,mm)和分布(Tb.Sp,μm)。对结果数据进行统计学分析发现,所有动物的aBMD均有减少。在髌腱切除组,经过LIPU治疗的胫骨aBMD没有显著降低(P<0.05),于神经切除组有减低(0.01<P<0.05)。在神经切除组中,经过LIPU治疗的骨小梁体积明显高于非治疗组(P<0.01)。骨小梁宽度和骨小梁分布也有相应的差异(0.01<P<0.05)。骨的形态学观察也有同样表现。由此可见,LIPU可以对防止废用性骨质疏松,尤其是无神经损伤的动物模型。This paper studies the therapeutic effects of Low-Intensity Pulsed Ultrasound （LIPU） on prevention of disused osteoporosis,by testing on tenotomy and neurectomy rats.A total of 40 male Sprague-Dawley rats were randomly assigned into four groups,each with 10 animals.They are tenotomy groups,which underwent left patellar-tenotomy,with/without ultrasound treatment and neurectomy groups,which underwent left sciatic-neurectomy,with/without ultrasound treatment.The ultrasound treatment groups were treated with 30mW/cm2 pulsed low-intensity ultrasound stimulation for 15 minutes a day on the lateral site of the left legs.The sham ultrasound groups （those without ultrasound treatment） did not receive any ultrasound treatment.All animals were euthanized after 4 weeks.Both tibias of rats were prepared for peripheral Quantitative Computed Tomography （pQCT）.Average Bone Mineral Density （aBMD） was measured.For the study of bone histomorphometry of proximal tibia,trabecular bone morphometry measurements were performed on the area beginning 1mm proximal to the growth plate （epiphysis）.Total tissue area,trabecular area,and perimeter were measured.These endpoints were used to calculate the trabecular bone volume （BV/TV,%）,trabecular width （Tb.Wi,μm）,trabecular number （Tb.N,mm） and trabecular separation （Tb.Sp,μm） according to the methods of Parfitt et al.Statistical analysis was performed by using SPSS 11.5 data analysis software.All data were expressed as the mean±SD,and difference were considered significant if P0.05.The decrease of aBMD in the operated tibia versus nonoperated tibia was 16%,and that was 17% in the neurotomy group.In ultrasound groups,aBMD did not decrease significantly in tenotomy group （P〉0.05）.But difference was found between operated and non-operated tibia in neurotomy group （0.01〈P〈0.05）.Moreover,in neurotomy groups,difference was found in bone loss percentage change between groups with and without ultrasound treatment.There was no difference in tenotomy

关 键 词：废用性骨质疏松症 低强度脉冲超声波 骨形态计量学 骨密度 

分 类 号：R580[医药卫生—内分泌]