血中苯妥英钠和苯巴比妥钠的高效毛细管电泳优化分析  

Advanced Analysis of Phenytoin Sodium and Phenobarbital Sodium in Blood Using High Performance Capillary Chromatography

作　　者：黄慧玲[1,2] 只达石[1,2] 周小东 

机构地区：[1]天津市神经外科研究所 [2]中国医科院基础医学研究所

出　　处：《分析测试学报》1999年第1期49-52,共4页Journal of Instrumental Analysis

摘　　要：建立了血中的苯妥英钠和苯巴比妥钠的胶束电动毛细管电泳分析方法（ＭＥＫＣ）。以２５ｍｍｏｌ／Ｌ硼酸钠溶液（ｐＨ＝８．６０）、３０ｍｍｏｌ／Ｌ十二烷基硫酸钠（胶束相）和甲醇３０％（φ）为缓冲液，恒定电压２５ｋＶ（电流３０～３５μＡ），检测波长２００ｎｍ，毛细管柱长３７ｃｍ。苯妥英钠和苯巴比妥钠两种药物质量浓度的线性范围为１．０～８０ｍｇ／Ｌ，最低检出质量浓度为１．０ｍｇ／Ｌ。该法为临床提供了一种高效。The determination of phenytoin sodium and phenobarbital sodium by micellar electrokinetic capillary chromatography(MEKC) with sodium dodecyl sulphate(SDS)as the micellar phase is described. The factors affecting the micellar electrokinetic separation were studied for quantitative determination of two components in standards and in blood. The optimal separation of two components was achieved with 25 mmol/L borate sudium buffer(pH 8.60 ),30 mmol/L SDS solution, 30% methanol as a buffer and a constant voltage of 25 kV(current of 30～35 μA). The method has a good linearity in the range of 1.0～80 mg/L of the drugs and detection limit of 1.0 mg/L. Thus, the electrokinetic capillary chromatography is demonstrated to be a new, rapid, highly effective method for analysing the drugs in human blood quantitatively.

关 键 词：苯妥英钠 苯巴比妥钠 血液 MEKC 

分 类 号：R971.6[医药卫生—药品] R446.11[医药卫生—药学]