测定心血管紧张素转化酶抑制剂体外抑制动力学的反相HPLC法  

Reversed_phase HPLC Determination of the in_vitro Inhibition Kinetics of Angiotensin_converting Enzyme Inhibitors

作　　者：陈河如[1,2] 徐杰诚[1,2] 

机构地区：[1]汕头大学化学系 [2]中国科学院上海有机化学研究所

出　　处：《分析测试学报》1999年第1期69-71,共3页Journal of Instrumental Analysis

摘　　要：报道一种用反相ＨＰＬＣ技术简便、有效地研究心血管紧张素转化酶抑制剂体外抑制动力学的新方法。该法利用马尿酰－甘氨酰－甘氨酸作酶底物。A simple reversed_phase HPLC method for the study of in_vitro inhibition kinetics of angiotensin_converting enzyme inhibitors is described. This sensitive method employs hippuryl _ glycyl _ glycine as a substrate and reversed_phase HPLC to monitor hippuric acid concentration changes with time. Thus,the mechanism of the enzyme reaction could be studied.

关 键 词：高血压 血管紧张素 转化酶 抑制剂 ACE HPLC 

分 类 号：R544.105[医药卫生—心血管疾病]