基于HPLC-MS的健康吸烟人群代谢组学研究  

HPLC-MS-based metabonomics for the shudy of healthy smoker's urine

作　　者：何智慧 王媛[2] 石先哲[2] 张凤霞[2] 许国旺[2] 练文柳 

机构地区：[1]湖南中烟工业有限责任公司技术中心,湖南长沙410007 [2]中国科学院大连化学物理研究所国家色谱研究分析中心,辽宁大连116023

出　　处：《化学研究与应用》2010年第6期716-720,共5页Chemical Research and Application

基　　金：湖南中烟工业有限责任公司项目资助

摘　　要：利用液相色谱-质谱(HPLC-MS)技术,分析了健康吸烟人群的尿样,和非吸烟人相比,吸烟后尿样的代谢组分种类和浓度发生了一定程度的改变。虽然吸烟尚未对受试人群的健康状态产生显著影响,但已表现出和非吸烟人群代谢状态的差别。通过多变量数据处理手段,一些能够体现这种差别的潜在生物标记物得到了定性。这些潜在标记物多为和脂肪酸和氨基酸代谢有关的小分子物质。HPLC-MS has been applied to the analysis of healthy smoker＇s urine samples.Compared with non-smoker＇s urine sample,the varieties and the concentration of the metabolites of smoker＇s urine had changed.It obtained from the study that smoke influenced the metabolic activity although it had no influence on human healthy.Some potential biomarkers were found by analysing data with multi-variate method.These biomarkers were related to the metabolism of fatty acids or amino acids.

关 键 词：液相色谱-质谱 代谢组学 化学计量学 尿样 吸烟 

分 类 号：O657.6[理学—分析化学]