白附子炮制前后双(5-甲酰基糠基)醚含量的测定  

Determination of Double(5-Formyl Furfuryl) Ether contents in Rhizoma Typhonii before and after processing

作　　者：周友红[1] 张红伟 张振凌[3] 

机构地区：[1]南阳市中心医院,河南南阳473000 [2]信阳市药检所,河南信阳464000 [3]河南中医学院,河南郑州450008

出　　处：《中华中医药学刊》2010年第6期1186-1188,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家"十一五"科技支撑计划项目(2006BAI09B06);河南省重大科技攻关项目(0422030700;0522030200)

摘　　要：目的:建立白附子双(5-甲酰基糠基)醚的含量测定方法,探讨炮制对白附子中双(5-甲酰基糠基)醚含量的影响。方法:采用RP-HPLC法,以AgilentHC-C18为色谱柱(5μm,250mm×4.6mm),流动相为甲醇-水(39∶61),检测波长为278nm,流速0.8mL.min-1,柱温25℃,进样量10μL。结果:双(5-甲酰基糠基)醚在0.00217～0.01953μg范围内线性关系良好(r=0.9990),平均加样回收率96.44%(RSD为1.09%)。白附子中双(5-甲酰基糠基)醚含量生品<加压法制品<药典法制品。结论:炮制能提高白附子中双(5-甲酰基糠基)醚含量。Objective：To establish a method for content determination of Double （5-Formyl Furfuryl） Ether contents in Rhizoma Typhonii. Effects of Processing on Double （5-Formyl Furfuryl） Ether on the Contents of Rhizoma Typhonii. Methods：The experimental condition of the RP-HPLC method was as follows：AgilentHC-C18 column（5μm,250×4.6mm）,with linear gradient elution using methanol and water（39：61）; The flow rate was 0.8mL/min; Detected wavelength was 278 nm. The column temperature was 25℃and sampling quantity as10μL. Results：Double （5-Formyl Furfuryl） Ether demonstrated good linear relationship in the range of 0.00217～0.01953μg（r=0.9990）. The average recovery rate was 96.44%with RSD of 1.09%. Double （5-Formyl Furfuryl） Ether contents in Rhizoma Typhonii of rawPressure Method ProductsPharmacopoeia Method Products. Conclusion：Processing Can Improve the Content of Double （5-Formyl Furfuryl） Ether contents in Rhizoma Typhonii.

关 键 词：白附子 炮制 双(5-甲酰基糠基)醚 

分 类 号：R285.5[医药卫生—中药学]