HPLC法测定止嗽立效丸中盐酸麻黄碱的含量  

HPLC Analysis of Eyhedrine Hydrochloride in Lisouzhixiao Pills

作　　者：刘文娟[1] 王静岩[2] 张虹[1] 甄会贤[1] 

机构地区：[1]山西生物应用职业技术学院,山西太原030031 [2]吉林油田总医院江北分院检验科,吉林松源138000

出　　处：《中华中医药学刊》2010年第6期1322-1323,共2页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:建立测定止嗽立效丸中盐酸麻黄碱含量的方法。方法:采用高效液相色谱法,色谱柱为Kro-masilC18柱(5μm,4.6mm×250mm);流动相:甲醇-水(1∶1),检测波长为210nm。结果:精密度及稳定性均良好;盐酸麻黄碱浓度在0.63～4.38μg/mL范围内,峰面积与浓度(μg·mL-1)呈良好的线性关系,相关系数为0.9999,平均回收率为99.6%,RSD为1.22%。结论:本方法简单、快速、方便。Objective：To establish a method for the determination of eyhedrine hydrochloride in Lisouzhixiao Pills.Methods：An HPLC metod was used with Kromasil C18 column（5μm,4.6mm×250mm）;The mobile phase was methanol-water（1：1）,with the detection wavelength at 210nm.Resultes：The precision and stability were fine.The linear correlations of eyhedrine hydrochloride were over the concentrations 0.63μg/mL to 4.38μg/mL （r=0.9999） respectively.The average recoveries of eyhedrine hydrochloride is （99.6±1.22）% respectively.Conclusion：The method was convenient and simple and fast.

关 键 词：止嗽立效丸 盐酸麻黄碱 HPLC 

分 类 号：R285[医药卫生—中药学]