高寒草甸矮嵩草对氮肥的生理响应  

Physiological response of Kobresia humilis to nitrogenous fertilizer in an alpine meadow

作　　者：李以康[1] 冉飞[2,3] 包苏科[1,3] 韩发[1] 周华坤[1] 林丽[1] 张法伟[1] 

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810008 [2]中国科学院成都生物研究所,四川成都610041 [3]中国科学院研究生院,北京100039

出　　处：《草业学报》2010年第3期240-244,共5页Acta Prataculturae Sinica

基　　金：国家科技攻关计划项目(2005BA901A20);国家重点基础研究发展计划(973计划)(2009CB421102)资助

摘　　要：通过叶面喷施尿素处理,研究了自然生长状态下矮嵩草生长季节生理特性的变化动态,以深入了解高寒植物对氮肥的生理响应。结果表明,施肥处理显著提高了生长初期和生长后期矮嵩草叶内超氧化物歧化酶(SOD)活性(P<0.05);谷胱甘肽(GSH)含量变化没有表现出明显的规律性;处理和对照样地可溶性糖含量都呈"U"字形变化,在6和9月含量高,多数时间处理样地叶内可溶性糖含量显著高于对照;可溶性蛋白的含量在7和8月显著高于对照样地,在6和9月显著低于对照样地;处理样地叶绿素a、b和总叶绿素含量高于对照样地(除8月外);超氧阴离子(O2.-)产生速率除7月外,其他月份都低于对照样地。处理总体上提高了矮嵩草叶抗氧化能力和渗透调节能力,促进了矮嵩草的生长。Fertilization is a major measure to enhance the biomass of plants.The physiological response of Kobresia humilis to nitrogenous fertilizer in an alpine meadow was studied.The fertilization treatment significantly enhanced the activity of SOD,while there was no obvious change trend of GSH content and a ＂U＂ change of the soluble sugar content under the treatment and control.The soluble sugar content was highest in June and September,being significantly higher in the treatment than in the control for most of the time.The soluble protein content in the treatment was greater than that in the control during July and August,and less in June and September.The contents of chlorophyll a and b,and of total chlorophyll were also greater than those in the controls （except in August）.The generation velocity of O2·-was lower in the treatment than in the control （except in July）.The treatment also led to an increase in biomass,height,and coverage of the community.In conclusion,nitrogenous fertilizer treatment enhanced antioxidant capacity and osmo-adjustment,resulting in growth promotion of K.humilis.

关 键 词：氮肥 抗氧化系统 矮嵩草 

分 类 号：S812[农业科学—草业科学]