血红素氧合酶-2在新生大鼠延髓的表达及意义  

Expression and significance of heme oxygenase-2 in the medulla oblongata of newborn rats

作　　者：何颖[1] 张洁[1] 郑煜[1] 丁艳[1] 周华[1] 唐玉红[1] 陈丽[1] 

机构地区：[1]四川大学华西基础医学与法医学院生理学教研室,四川成都610041

出　　处：《西部医学》2010年第6期985-986,990,共3页Medical Journal of West China

基　　金：国家自然科学基金资助项目(NO.30770798)

摘　　要：目的探讨产生内源性一氧化碳(carbon monoxide,CO)的关键酶血红素氧合酶-2(heme oxygenase-2,HO-2)在新生大鼠延髓的表达及意义。方法采用新生Sprague-Dawley大鼠,行石蜡切片,分别进行HE染色和免疫组织化学染色(SP法),观察新生大鼠延髓神经核团的分布和不同神经核团中神经元的形态,以及新生大鼠延髓是否存在HO-2阳性神经元和阳性神经元的分布情况。结果在舌下神经核、疑核、巨细胞网状核、面神经核等部位均可见HO-2阳性神经元。结论 HO-2阳性神经元存在于新生大鼠延髓呼吸相关神经核团,提示内源性CO可能参与中枢性呼吸活动的调节。Objective To test the expression and significance of heme oxygenase-2（HO-2）,a key enzyme of synthesizing carbon monoxide（CO）,in the medulla oblongata of newborn rats.Methods The experiments were carried out on the medullary slices of newborn Sprague-Dawley rats with paraffin section,the distribution of nuclei and the morphology of neurons in the medulla oblongata of newborn rats were observed with HE staining,the expression of HO-2 in the medulla oblongata of newborn rats was investigated with the immunohistochemical staining.Results HO-2-positive immunoreactive neurons could be observed in hypoglossal nucleus,ambiguous nucleus,gigantocellular reticular nucleus and facial nucleus in the medulla oblongata of newborn rats.Conclusion HO-2-positive immunoreactive neurons exist in the medullary respiratory related nuclei of newborn rats,which suggests that endogenous CO may be involved in the regulation of central respiratory activity.

关 键 词：血红素氧合酶-2 一氧化碳 新生大鼠 延髓 呼吸调节 

分 类 号：R338.2[医药卫生—人体生理学] Q24[医药卫生—基础医学]