姜黄素对胶原诱导性关节炎大鼠滑膜细胞增殖的影响  

Effects of Curcumin on Proliferation of Synoviocytes in Arthritic Rats Induced by TypeⅡ Collagen

作　　者：朱丽艳[1] 李颖[1] 王建辉[1] 杨林[1] 

机构地区：[1]河北省唐山华北煤炭医学院基础部机能实验室,063000

出　　处：《青岛医药卫生》2010年第3期161-163,共3页Qingdao Medical Journal

基　　金：河北省教育厅自然科学研究计划项目;编号:2004119

摘　　要：目的探讨姜黄素对胶原诱导性关节炎(CIA)大鼠滑膜细胞的影响。方法将雄性Wistar大鼠皮内注射Ⅱ型胶原,建立CIA大鼠模型,取其滑膜组织用胶原酶消化后提取滑膜细胞进行原代培养,取3-5代的滑膜细胞进行后续实验。应用MTT比色法检测滑膜细胞的增殖水平。结果 5、10、20、40、80、160μmol/L的姜黄素作用24、48和72小时,均能明显抑制CIA大鼠滑膜细胞的增殖(P<0.05),且具有时间、剂量依赖性。结论姜黄素能明显抑制CIA大鼠滑膜细胞的增殖并呈时间、剂量依赖性改变。Objective To investigate the effects of curcumin on synovial cells of collagen-induced arthritis（CIA）rats. Methods Male Wistar rats were intradermal injected type Ⅱ collagen to establish CIA rat model. Synovial cells were extracted from synovial tissue after collagenase digestion. 3 to 5 generations of synovial cells were selected in follow-up experiments. MTT was used to detect proliferation level of synovial cells. Results 5,10,20,40,80,160μmol/L of curcumin could inhibit the proliferation of synovial cells（P〈0. 05）after 24,48 and 72 hours,and had time-and dose-dependent. Conclusion Curcumin can sig- nificantly inhibit the synovial cells proliferation of CIA rats,and the relationship shows time-and dose-dependent.

关 键 词：姜黄素 胶原诱导性关节炎 类风湿性关节炎 滑膜细胞 

分 类 号：R285.5[医药卫生—中药学]