E838及其联合放化疗对IRM-2小鼠自发淋巴瘤的抑制作用  

Inhibitive effects of E838 combined radiotherapy and chemotherapy on malignant tumor in IRM-2 mice

作　　者：王月英[1] 李德冠[1] 吴红英[1] 张恒[1] 王小春[1] 杜利清[1] 路璐[1] 孟爱民[1] 

机构地区：[1]中国医学科学院中国协和医科大学放射医学研究所天津市分子核医学重点实验室,天津300192

出　　处：《中国生化药物杂志》2010年第3期154-157,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金(30770645);天津市自然科学重点基金项目(08JCYBJC07300)

摘　　要：目的观察E838及其联合放化疗对IRM-2小鼠自发淋巴瘤的抑制作用及对骨髓有核细胞数的影响。方法放疗组采取2～3 mm3瘤块接种于小鼠腋部皮下。化疗组采用1∶3(生理盐水)比例制成细胞悬液,每只小鼠前肢腋窝下皮下接种0.2 mL,建立小鼠肿瘤模型。放疗组在24 h后将荷瘤小鼠随机分为对照组、单放组、E838100,150,200μg/0.2 mL(低、中、高)治疗组及药物合用照射组;化疗组分为对照组、环磷酰胺(CTX)组、E838低、中、高治疗组及合用CTX组。治疗组连续7 d腹腔注射给药,合用照射组于给药的第4天进行全身137Csγ射线1Gy照射,1次/d,连续5 d。实验第12天时处死小鼠,剥离瘤体测瘤重,计算抑瘤率。合用化疗组于24 h后开始给药(CTX 500μg/0.2 mL),隔日1次,共4次。实验第12天处死小鼠,剥离瘤体测瘤重,计算抑瘤率。结果 E838(低、中、高)合用137Csγ射线能提高抑瘤效果,抑瘤率分别为65.43%,77.13%和67.55%,比E838治疗组(低、中、高)分别升高21.29%,6.39%,17.55%,抑制率比照射组提高42.83%,差异有统计学意义(P<0.001)。q值分别为1.03,0.95和1.00,显示与γ射线合用有相加作用。合用CTX的抑瘤率分别为81.68%,82.70%,82.14%,比治疗组分别升高42.9%,36.19%,40.05%,抑制率比CTX组提高21.0%,差异有统计学意义(P<0.001)。q值分别为1.07,1.04和1.06,与CTX合用有相加作用。结论 E838对IRM-2小鼠自发淋巴瘤具有抑制作用,E838联合放化疗能提高抑瘤效果。Purpose To observe the inhibitive effects of E838 combined radiotherapy and chemotherapy on malignant anti-tumor and the effects on the number of bone marrow nucleated cells. Methods - Establishment of mouse tumor model： to the radiotherapy group mice,2-3 mm^3 tumors inoculated subcutaneously in the axillary region, to the chemotherapy group mice （NS） ,0.2 mL cell suspension （diluted with normal saline to the ratio of 1 to 3） were inoculated subcutaneously in forelimb armpit region. After 24 h of the establishment, the radiotherapy group mice were randomly divided into control group, radiotherapy alone group,low, middle and high dose E838 combined treatment group and drug combined irradiation group, the chemotherapy group was divided into control group, cyclophosphamide （CTX） group, low, medium,high dose E838 combined treatment group and CTX combined treatment group, CTX combined treatment group began administrating drugs（ CTX 500 μg/0.2 mL） ,The drugs need to be injected 4 times every 2 days. In the treatment group drugs were intraperitoneal injected for 7 d, and mice in drug combined irradiation group were total-body irradiated by 1Gy ^137 Cs μ-ray daily for 5 days from the 4th day of administration. On the 12 days, the mice were sacrificed and mice tumor were weighted and oncostatie rate was calculated. Results E838 combined ^137 Cs μ-ray was a better tumor suppressor effect and the inhibi- tion ratio was 65.43% ,77.13% and 67.55% respectively. The inhibition ratio increased by 21.29% ,6. 39% and 17.55% compared with the treatment group and increased by 42.83% compared with the control group and there were significant differences （P 〈 0. 001 ）. The q values were 1.03,0.95 and 1.00 respectively which showed that there were synergistic antitumor effects combined with radiotherapy. In E838 combined CTX group, the inhibition ratio was 81.68% ,82.70% and 82.14% respectively. The inhibition ratio increased by 42.9% ,36. 19% and 40.05% compared with the treatment group and increased by 21.0

关 键 词：E838 恶性肿瘤 放射治疗 化学治疗 IRM-2小鼠 

分 类 号：R965[医药卫生—药理学]