壳聚糖絮凝法澄清复方黄芪水提液的工艺研究  

Clarification process of compound astragalus aqueous extract with chitosan

作　　者：贺培益[1,2] 王米[2] 孟新宇[2] 杨锐乐[2] 江善祥[1] 薛飞群[2] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]中国农业科学院上海兽医研究所药学研究室,上海200241

出　　处：《中国生化药物杂志》2010年第3期173-175,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家"十一.五"科技支撑计划"中兽药添加剂的研制与开发"子专题(2008BADB4B07-3)

摘　　要：目的优选最佳的复方黄芪水提液的壳聚糖澄清工艺。方法经预试验确定澄清剂的大致剂量,用苯酚-硫酸法测定多糖含量,以药液絮凝度和多糖含量为指标,以壳聚糖加入量、絮凝温度、絮凝时间为考察因素,采用正交试验摸索最优的壳聚糖絮凝条件。结果得到了最优的絮凝工艺,即药液中壳聚糖最佳用量为2 g/L,20℃的温度下絮凝24 h。结论壳聚糖絮凝法简便易行,经济实用,制剂澄清效果好。Purpose To optimize the clarification process of compound astragalus aqueous extract with ehitosan. Methods The content of ploysaccharides was determined by phenol-sulfuric acid method with ultraviolet spectrophotometer,and the orthogonal design was used to study the three factors,including the mount of chitosan solution added, the flocculation temperature and time, taking level of flocculation and the content of polysaeeharides as markers. Results The optimal clarification procedure was obtained. Under these conditions,the concentration of chitosan was 0.2% and the temperature of flocculation was 20℃ for 24 h. Conclusion The preparation shows a good clarity and the method is feasible and economical.

关 键 词：多糖 壳聚糖 苯酚-硫酸法 正交试验 

分 类 号：R284.2[医药卫生—中药学]